Rabbit anti-Human MLLT10 Polyclonal Antibody | anti-MLLT10 antibody

MLLT10, CT (AF10) (Protein AF-10, AF10, ALL1-fused Gene From Chromosome 10 Protein) (PE)

Cat. Num. AAA6461862

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MLLT10; Polyclonal Antibody; CT (AF10) (Protein AF-10; AF10; ALL1-fused Gene From Chromosome 10 Protein) (PE); anti-MLLT10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLLT10.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1027

• Applicable Applications for anti-MLLT10 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MLLT10 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MLLT10 Pab in Jurkat cell line lysate. MLLT10(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with MLLT10 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MLLT10 antibody

Antibodies; Disease Markers

References

1. Perrin, L., et al., Mol. Cell. Biol. 23(1):119-130 (2003). 2. Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002). 3. Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002). 4. Chaplin, T., et al., Blood 85(6):1435-1441 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4757726
• NCBI Accession #: NP_004632
• NCBI GenBank Nucleotide #: NP_004632.1
• UniProt Accession #: P55197
• Molecular Weight: 109.026
• NCBI Official Full Name: protein AF-10 isoform a
• UniProt Protein Name: Protein AF-10
• Protein Family: Protein
• UniProt Gene Name: MLLT10
• UniProt Synonym Gene Names: AF10
• UniProt Entry Name: AF10_HUMAN

Uniprot Description

MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; DNA binding; zinc ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

Disease: Leukemia, Acute Myeloid