Rabbit MLL5 Polyclonal Antibody | anti-KMT2E antibody

MLL5 Antibody

Cat. Num. AAA9629107

• Gene Names: KMT2E; MLL5; NKp44L; HDCMC04P
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MLL5; Polyclonal Antibody; MLL5 Antibody; HDCMC04P; Histone lysine N methyltransferase MLL5; Histone-lysine N-methyltransferase MLL5; KMT2E; Lysine N methyltransferase 2E; Lysine N-methyltransferase 2E; MGC70452; Mll5; MLL5_HUMAN; Myeloid/lymphoid or mixed lineage leukemia 5 (trithorax homolog; Drosophila); Myeloid/lymphoid or mixed lineage leukemia 5; Myeloid/lymphoid or mixed lineage leukemia protein 5; Myeloid/lymphoid or mixed-lineage leukemia protein 5; anti-KMT2E antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MLL5 Antibody detects endogenous levels of total MLL5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KMT2E antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The PHD-type domain binds specifically histone H3 tri-methylated at 'Lys-4' (H3K4me3), thus promoting binding to chromatin. The SET domain does not bind the methyl group donor S-adenosyl-L-methionine and histone 3 H3K4 peptide as a large loop prevents the docking of the 'Lys-4' side chain. The C-terminus domain is responsible for the localization to the centrosome during mitosis. Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
• Post Translational Modifications: Ubiquitinated. Deubiquitinated by USP7.O-glycosylated at Ser-435 and Thr-440 in the SET domain by OGT which probably prevents KMT2E proteasomal-mediated degradation.
• Subunit Structure: Component of a complex composed of KMT2E (isoform 3), OGT and USP7; the complex stabilizes KMT2E, preventing KMT2E ubiquitination and proteosomal-mediated degradation. Isoform 3 interacts (via N-terminus) with OGT (via TRP repeats). Isoform 3 interacts with deubiquitinating enzyme USP7 (via MATH domain). Isoform 3 interacts (via HBM motif) with HCFC1 (via Kelch domain). Isoform 3 interacts with E2F1; the interaction is probably indirect and is mediated via HCFC1.
• Tissue Specificity: Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum. Isoform NKp44L: Not detected on circulating cells from healthy individuals, but is expressed on a large panel of tumor and transformed cells.
• Function: Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription. Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (By similarity). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry. Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition. During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (By similarity).Cellular ligand for NCR2/NKp44, may play a role as a danger signal in cytotoxicity and NK-cell-mediated innate immunity.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KMT2E antibody

Chromosome; Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Nucleus speckle; Primary; Peripheral membrane protein

NCBI and Uniprot Product Information

• NCBI GI #: 91199543
• NCBI GeneID: 55904
• NCBI Accession #: NP_061152.3
• NCBI GenBank Nucleotide #: NM_018682.3
• UniProt Accession #: Q8IZD2; Q6P5Y2; Q6PKG4; Q6T316; Q86TI3; Q86W12; Q86WG0; Q86WL2; Q8IV78; B6ZDE4; B6ZDM3; M4K8J3
• Molecular Weight: 131,737 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase 2E
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2E
• NCBI Official Symbol: KMT2E
• NCBI Official Synonym Symbols: MLL5; NKp44L; HDCMC04P
• NCBI Protein Information: histone-lysine N-methyltransferase 2E; histone-lysine N-methyltransferase MLL5; myeloid/lymphoid or mixed-lineage leukemia protein 5; myeloid/lymphoid or mixed-lineage leukemia 5 (trithorax homolog, Drosophila)
• UniProt Protein Name: Histone-lysine N-methyltransferase 2E
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: KMT2E
• UniProt Synonym Gene Names: MLL5; Lysine N-methyltransferase 2E
• UniProt Entry Name: KMT2E_HUMAN

NCBI Description

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

MLL5: Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Transcription, coactivator/corepressor; Methyltransferase; Cell cycle regulation; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; intercellular bridge; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nuclear speck

Molecular Function: protein binding; enzyme binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity

Biological Process: retinoic acid receptor signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; neutrophil activation; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; histone H3-K4 methylation; DNA methylation; neutrophil mediated immunity; erythrocyte differentiation; cell cycle arrest