Mouse anti-Human CD131 Monoclonal Antibody | anti-CD131 antibody

Anti-CD131 [BION-1], Rabbit IgG, Kappa

Cat. Num. AAA488509

• Gene Names: CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot, Immunohistochemistry
• Purity: Protein A affinity purified
• Synonyms: CD131; Monoclonal Antibody; Anti-CD131 [BION-1]; Rabbit IgG; Kappa; CSF2RB; IL3RB; IL-3R common beta subunit; IL5RB; colony stimulating factor 2 receptor; beta; low-affinity (granulocyte-macrophage); SMDP5; Common Beta-Chain; CDw131; cytokine receptor common subunit beta; beta-c; Beta Common Chain; anti-CD131 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, Kappa
• Clone Number: BION-1
• Specificity: Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD131 antibody: Blocking (BL), Crystallisation, Functional Studies, Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun 1999). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom 2002). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn 200).
• Antibody Type: Recombinant Antibody
• Immunogen: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of U937 cells using anti-CD131 antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow-cytometry using the Anti-CD131 antibody BION-1 or the rabbit IgG version of BION-1 ( at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-CD131 antibody

Describes the presence of the common beta-chain in various cell surface receptors and its role in receptor activation for inflammation. The use of BION-1 as an antagonist of IL-5, GM-CSF and IL-3 signalling through binding the common beta-chain is also outlined in the context of blocking eosinophil activation.
Recombinant monoclonal antibody to CD131. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma BION-1.

Product Categories/Family for anti-CD131 antibody

Immunology

References

Sun et al.; Blood, Vol 94, No 6 (September 15), 1999: pp 1943-1951. OriginPubPMID: 10753826

NCBI and Uniprot Product Information

• NCBI GI #: 4559408
• NCBI GeneID: 1439
• NCBI Accession #: NP_000386.1
• NCBI GenBank Nucleotide #: NM_000395.2
• UniProt Accession #: P32927; Q5JZI1; Q6ICE0
• Molecular Weight: 97,336 Da
• NCBI Official Full Name: cytokine receptor common subunit beta
• NCBI Official Synonym Full Names: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
• NCBI Official Symbol: CSF2RB
• NCBI Official Synonym Symbols: CD131; IL3RB; IL5RB; SMDP5; CDw131
• NCBI Protein Information: cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta-chain; GM-CSF/IL-3/IL-5 receptor common beta subunit; colony-stimulating factor-2 receptor, beta, low-affinity; interleukin 3 receptor/granulocyte-macrophage colony stimulating f
• UniProt Protein Name: Cytokine receptor common subunit beta
• UniProt Gene Name: CSF2RB
• UniProt Synonym Gene Names: IL3RB; IL5RB
• UniProt Entry Name: IL3RB_HUMAN

NCBI Description

The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]

Uniprot Description

CSF2RB: a common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. A type I cytokine family of receptor. Defects are a cause of congenital pulmonary alveolar proteinosis (PAP) [MIM:265120]. PAP is an autosomal recessive fatal respiratory disease.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: granulocyte macrophage colony-stimulating factor receptor complex; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; interleukin-5 receptor activity; interleukin-3 receptor activity; receptor activity

Biological Process: response to lipopolysaccharide; respiratory gaseous exchange; signal transduction

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 5