Rabbit MLK3 Polyclonal Antibody | anti-MLK3 antibody

MLK3 (Phospho-T277/S281) Polyclonal Antibody

Cat. Num. AAA3007867

• Gene Names: MAP3K11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MLK3; Polyclonal Antibody; MLK3 (Phospho-T277/S281) Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase; MAP3K11; PTK1; SPRK; anti-MLK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-MLK3 (T277/S281) polyclonal antibody detects endogenous levels of human MLK3 when phosphorylated at Thr602 and Ser281.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 847

• Applicable Applications for anti-MLK3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human MLK3 around the phosphorylation site of Threonine 277 and Serine281.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-MLK3 (T277/S281) polyclonal antibody at 1:500 dilutionLane1:HEK293Twhole cell lysateLane2:NIH-3T3 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-MLK3 antibody

As a result of the binding of growth factors to their membrane receptors, cytoplasmic proteins containing Src homology 2 (SH2) domains associate with specific phosphotyrosine residues within the activated receptors and function as signaling intermediates. The action of such SH2 domain proteins frequently involves the activation of a second group of signaling intermediates characterized by SH3 domains. These latter proteins function through binding proline-rich sequences in target proteins. A novel human non-receptor protein kinase, designated either MLK3 or PTK1, is 847 amino acids in length and contains an SH3 domain in the absence of an SH2 domain. In addition, MLK3 is characterized by a leucine zipper basic region (a motif commonly associated with transcription factors) and has a long carboxy-terminal tail which exhibits proline-rich motifs similar to known SH3 binding sites. MLK3 is expressed widely and is related to the previously described MLK1 and MLK2 kinases.

NCBI and Uniprot Product Information

• NCBI GI #: 71153819
• NCBI GeneID: 4296
• UniProt Accession #: Q16584
• Molecular Weight: ~ 93, 100kDa
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 11
• NCBI Official Symbol: MAP3K11
• NCBI Official Synonym Symbols: MLK3; PTK1; SPRK; MLK-3; MEKK11
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 11
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 11
• UniProt Gene Name: MAP3K11
• UniProt Entry Name: M3K11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]

Uniprot Description

MLK3: a TKL kinase of the MLK family. Has an amino-terminal SH3 domain followed by the kinase domain, two leucine zipper domains, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. Phosphorylates IkappaB and activates SEK1 and MKK7.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily

Chromosomal Location of Human Ortholog: 11q13.1-q13.3

Cellular Component: centrosome; cytoplasm; membrane; microtubule

Molecular Function: ATP binding; identical protein binding; JUN kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; Rac GTPase binding

Biological Process: activation of JNK activity; activation of JNKK activity; activation of MAPK activity; cell death; cell proliferation; JNK cascade; microtubule-based process; peptidyl-tyrosine phosphorylation; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of neuron apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation