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Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody.)

Rabbit anti-Human MLC2 Polyclonal Antibody | anti-MLC2 antibody

MLC2 (Phospho-Tyr118) Antibody

Gene Names
MYL9; LC20; MLC2; MRLC1; MYRL2; MLC-2C
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
MLC2; Polyclonal Antibody; MLC2 (Phospho-Tyr118) Antibody;  MRLC1; MYRL2; anti-MLC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of MLC2 only when phosphorylated at tyrosine 118.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
172
Applicable Applications for anti-MLC2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500~1:1000
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y(p)-V-R) derived from Human MLC2.
Modification
Phospho-Tyr118
Target Name
MLC2
Preparation and Storage
Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody.)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed U87 cells using MLC2 (Phospho-Tyr118) Antibody.)

Testing Data

Testing Data
Related Product Information for anti-MLC2 antibody
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Product Categories/Family for anti-MLC2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,866 Da
NCBI Official Full Name
myosin regulatory light polypeptide 9 isoform a
NCBI Official Synonym Full Names
myosin, light chain 9, regulatory
NCBI Official Symbol
MYL9
NCBI Official Synonym Symbols
LC20; MLC2; MRLC1; MYRL2; MLC-2C
NCBI Protein Information
myosin regulatory light polypeptide 9
UniProt Protein Name
Myosin regulatory light polypeptide 9
Protein Family
UniProt Gene Name
MYL9
UniProt Synonym Gene Names
MLC2; MRLC1; MYRL2; LC20
UniProt Entry Name
MYL9_HUMAN

NCBI Description

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: stress fiber; muscle myosin complex; Z disc; cytosol

Molecular Function: structural constituent of muscle; calcium ion binding

Biological Process: axon guidance; muscle contraction; regulation of muscle contraction; ephrin receptor signaling pathway

Research Articles on MLC2

Similar Products

Product Notes

The MLC2 myl9 (Catalog #AAA9401796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLC2 (Phospho-Tyr118) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the MLC2 myl9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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