Testing Data
(3T3/A31 cell lysate was probed with M4110-01 (0.3ug/ml).)
Rabbit anti-Human, Mouse MKP 1 Polyclonal Antibody | anti-MKP1 antibody
MKP 1 (Mitogen-activated Protein Kinase Phosphatase 1, MAP Kinase Phosphatase 1, MKP1, MKP-1, CL100, Dual Specificity Protein Phosphatase 1, DUSP1, Dual Specificity Protein Phosphatase hVH1, HVH1, Protein-tyrosine Phosphatase CL100, PTPN10, VH1) (AP)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MKP 1; Polyclonal Antibody; MKP 1 (Mitogen-activated Protein Kinase Phosphatase 1; MAP Kinase Phosphatase 1; MKP1; MKP-1; CL100; Dual Specificity Protein Phosphatase 1; DUSP1; Dual Specificity Protein Phosphatase hVH1; HVH1; Protein-tyrosine Phosphatase CL100; PTPN10; VH1) (AP); Dual Specificity Phosphatase 1; EC=3.1.3.16; EC=3.1.3.48; anti-MKP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MKP1, Mr ~42kD. Species Crossreactivity: mouse. Species Sequence Homology: rat
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-MKP1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
WB: 0.1-1ug/ml detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.3ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. MKP1 (~42kD).
WB: 0.1-1ug/ml detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.3ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. MKP1 (~42kD).
Immunogen
Synthetic peptide corresponding to aa87-101, DERSAALDGAKRDGT-C of human MAP kinase phosphatase 1 (MKP1) conjugated to KLH, C-terminal cysteine added for conjugation purposes.
Conjugate
AP
Included Positive Antigen Control
MBS657179: Fibroblast Cell, 3T3/A31 lysate. Add 2.5ul 2-mercaptoethanol/100ul of lysate. Boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for Western Blot analysis.
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Testing Data
(3T3/A31 cell lysate was probed with M4110-01 (0.3ug/ml).)
Testing Data
(3T3/A31 cell lysate was probed with M4110-01 (0.3ug/ml).)
Product Categories/Family for anti-MKP1 antibody
References
1. Zhang, C.F. and K.L. Guan, J. Biol. Chem. 268: 16116-16119, 1993. 2. Lasa, M., et al., Mol. Cell. Biol. 22: 7802-7811, 2002. 3. Imasato, A., et al., J. Biol. Chem. 277: 47444-47450, 2002.
Similar Products
Product Notes
The MKP1 (Catalog #AAA6488345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MKP 1 (Mitogen-activated Protein Kinase Phosphatase 1, MAP Kinase Phosphatase 1, MKP1, MKP-1, CL100, Dual Specificity Protein Phosphatase 1, DUSP1, Dual Specificity Protein Phosphatase hVH1, HVH1, Protein-tyrosine Phosphatase CL100, PTPN10, VH1) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MKP 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 0.1-1ug/ml detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with.3ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. MKP1 (~42kD). Researchers should empirically determine the suitability of the MKP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKP 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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