Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-MKK7 (Ser271) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 62kDa)
Rabbit MKK7 Polyclonal Antibody | anti-MKK7 antibody
Phospho-MKK7 (Ser271) Antibody
Tissue Specificity: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-MKK7 (Ser271) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 62kDa)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-MKK7 (Ser271) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 62kDa)
Immunohistochemistry (IHC)
(Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]
Uniprot Description
MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.
Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Kinase, protein; STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: cytoplasm; intracellular; cytosol; nucleus
Molecular Function: MAP kinase kinase activity; protein C-terminus binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; protein kinase binding; protein phosphatase binding; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; response to osmotic stress; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to heat; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; response to UV
Research Articles on MKK7
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Product Notes
The MKK7 map2k7 (Catalog #AAA9612928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MKK7 (Ser271) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MKK7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). IHC: 1:50-1:200 WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the MKK7 map2k7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKK7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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