Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cels.)
Rabbit anti-Human Mitogen Activated Protein Kinase Kinase 2 Polyclonal Antibody | anti-MAP2K2 antibody
Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 2 (MAP2K2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VTKVQ HRPSGLIMAR KLIHLEIKPA IRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMAPERLQ GTHYSVQSDI WSMGLSLVEL AVGRYPIPPP DAKELEAIFG
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cels.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat cells; Lane2: Human Hela cels.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MAP2K2, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MAP2K2, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: MAP2K2 knockout Hela cell lysate; Predicted MW: 44kd Observed MW: 44kd Primary Ab: 5ug/ml Rabbit Anti-Human MAP2K2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: MAP2K2 knockout Hela cell lysate; Predicted MW: 44kd Observed MW: 44kd Primary Ab: 5ug/ml Rabbit Anti-Human MAP2K2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasmic side of plasma membrane; cytosol; early endosome; endoplasmic reticulum; extracellular region; focal adhesion; Golgi apparatus; intercellular junction; late endosome; microtubule; mitochondrion; nucleus; perinuclear region of cytoplasm; peroxisomal membrane; plasma membrane
Molecular Function: ATP binding; MAP kinase kinase activity; metal ion binding; PDZ domain binding; protein binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity
Biological Process: activation of MAPK activity; ERK1 and ERK2 cascade; MAPK cascade; negative regulation of gene expression; peptidyl-tyrosine phosphorylation; positive regulation of ERK1 and ERK2 cascade; positive regulation of protein serine/threonine kinase activity; positive regulation of transcription, DNA-templated; regulation of apoptosis; regulation of early endosome to late endosome transport; regulation of Golgi inheritance; regulation of mitotic cell cycle; regulation of stress-activated MAPK cascade
Disease: Cardiofaciocutaneous Syndrome 4
Research Articles on MAP2K2
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Product Notes
The MAP2K2 map2k2 (Catalog #AAA2027140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 2 (MAP2K2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mitogen Activated Protein Kinase Kinase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MAP2K2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VTKVQ HRPSGLIMAR KLIHLEIKPA IRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEEIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMAPERLQ GTHYSVQSDI WSMGLSLVEL AVGRYPIPPP DAKELEAIFG. It is sometimes possible for the material contained within the vial of "Mitogen Activated Protein Kinase Kinase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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