Rabbit anti-Rat Mitogen Activated Protein Kinase 8 (MAPK8) Polyclonal Antibody | anti-MAPK8 antibody

Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8)

Cat. Num. AAA2004200

• Gene Names: Mapk8; JNK
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Mitogen Activated Protein Kinase 8 (MAPK8); Polyclonal Antibody; Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8); anti-MAPK8 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 0.44mg/ml (varies by lot)

• Applicable Applications for anti-MAPK8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-2 ug/mL;1:220-880; Immunohistochemistry: 5-20 ug/mL;1:22-88; Immunocytochemistry: 5-20 ug/mL;1:22-88; ; Optimal working dilutions must be determined by end user.
• Organism Species: Rattus norvegicus (Rat)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant JNK1 (Gly138~Gly370 (Accession # P49185)) expressed in E.coli
• Cross Reactivity: Rat
• Preparation and Storage: Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 12 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant MAPK8 , Rat)

Immunohistochemistry (IHC)

(DAB staining on IHC-PSamples: Rat Small intestine TissuePrimary Ab: 20 ug/ml Rabbit Anti-Rat MAPK8 AntibodySecond Ab: 2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC)

(DAB staining on IHC-PSamples: Rat Testis Tissue Primary Ab: 20 ug/ml Rabbit Anti-Rat MAPK8 Antibody Second Ab: 2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

• NCBI GI #: 694879445
• NCBI GeneID: 116554
• NCBI Accession #: NP_446281.2
• NCBI GenBank Nucleotide #: NM_053829.2
• UniProt Accession #: P49185
• NCBI Official Full Name: mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: Mapk8
• NCBI Official Synonym Symbols: JNK
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: Mapk8
• UniProt Synonym Gene Names: Jnk1; Prkm8; MAP kinase 8; MAPK 8

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. [provided by RefSeq, Jul 2012]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: CMGC group; EC 2.7.11.24; JNK subfamily; Kinase, protein; MAPK family; MAPK/JNK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p16

Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; intracellular; mitochondrion; nucleus; perikaryon; podosome; vesicle

Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; MAP kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cellular response to amino acid starvation; cellular response to amyloid-beta; cellular response to cadmium ion; cellular response to hydrogen peroxide; cellular response to interleukin-1; cellular response to nitric oxide; cellular response to reactive oxygen species; dendrite morphogenesis; inflammatory response; JNK cascade; JUN phosphorylation; MAPK cascade; negative regulation of apoptosis; negative regulation of protein binding; neuron development; neuron migration; neuron projection development; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; positive regulation of apoptotic signaling pathway; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of deacetylase activity; positive regulation of determination of dorsal identity; positive regulation of DNA replication; positive regulation of gene expression; positive regulation of microtubule polymerization; positive regulation of neuroblast proliferation; positive regulation of neuron apoptotic process; positive regulation of neuron migration; positive regulation of podosome assembly; positive regulation of protein metabolic process; programmed necrotic cell death; protein amino acid phosphorylation; protein localization to tricellular tight junction; regulation of centrosome cycle; regulation of circadian rhythm; regulation of DNA replication origin binding; regulation of gene expression; regulation of histone deacetylation; regulation of neuron differentiation; regulation of protein localization; regulation of transcription, DNA-templated; response to cadmium ion; response to heat; response to hydrogen peroxide; response to mechanical stimulus; response to osmotic stress; response to steroid hormone; response to UV; rhythmic process; signal transduction; stress-activated MAPK cascade