Rabbit anti-Human Mitofusin 2 Polyclonal Antibody | anti-MFN2 antibody

Mitofusin 2 Antibody: Biotin

Cat. Num. AAA8000042

• Gene Names: MFN2; HSG; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B
• Reactivity: Human
• Applications: Western Blot
• Purity: Peptide Affinity Purified
• Synonyms: Mitofusin 2; Polyclonal Antibody; Mitofusin 2 Antibody: Biotin; Rabbit Anti-Human Mitofusin 2 Polyclonal; Transmembrane GTPase MFN2 Antibody; CPRP1 Antibody; MFN2 Antibody; Mitofusin-2 Antibody; EC:3.6.5.- Antibody; KIAA0214 Antibody; anti-MFN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects 86.4 kDa.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 757

• Applicable Applications for anti-MFN2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Target: Mitofusin 2
• Immunogen: Synthetic peptide from the mid-protein of Human MFN2
• Immunogen Species: Human
• Cellular Localization: Mitochondrion Outer Membrane; Multi-Pass Membrane Protein
• Conjugate: Biotin
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Mitofusin 2 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Mitofusin 2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

Western Blot (WB)

(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~86.4 kDa Mitofusin 2 Antibody protein using Rabbit Anti-Mitofusin 2 Antibody Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Embryonic kidney epithelial cell line (HEK293T) lysate. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Mitofusin 2 Antibody Polyclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~86.4 kDa.)

Product Categories/Family for anti-MFN2 antibody

Antibodies; Cell Signaling; Neuroscience; Neurodegeneration; Cell Markers; Cancer; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 189083768
• NCBI GeneID: 9927
• NCBI Accession #: NP_001121132.1
• NCBI GenBank Nucleotide #: NM_001127660.1
• UniProt Accession #: O95140; O95572; Q5JXC3; Q5JXC4; Q9H131; Q9NSX8; A8K1B3
• Molecular Weight: 50,041 Da
• NCBI Official Full Name: mitofusin-2
• NCBI Official Synonym Full Names: mitofusin 2
• NCBI Official Symbol: MFN2
• NCBI Official Synonym Symbols: HSG; MARF; CMT2A; CPRP1; CMT2A2; HMSN6A; CMT2A2A; CMT2A2B
• NCBI Protein Information: mitofusin-2
• UniProt Protein Name: Mitofusin-2
• Protein Family: Mitofusin
• UniProt Gene Name: MFN2
• UniProt Synonym Gene Names: CPRP1; KIAA0214

NCBI Description

This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:11181170, PubMed:11950885, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:28114303). Overexpression induces the formation of mitochondrial networks (PubMed:28114303). Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (). Plays an important role in the regulation of vascular smooth muscle cell proliferation (). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (PubMed:23620051). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions ().