Rabbit anti-Human KIT Polyclonal Antibody | anti-KIT antibody

KIT, CT (S821/Y823) (Kit, Sl, Mast/stem cell growth factor receptor Kit, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117) (PE)

Cat. Num. AAA6313500

• Gene Names: KIT; PBT; SCFR; C-Kit; CD117
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KIT; Polyclonal Antibody; CT (S821/Y823) (Kit; Sl; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; CD117) (PE); Kit; CD117; anti-KIT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KIT antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 807-836 amino acids from the C-terminal region of human KIT.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KIT Antibody (C-term S821/Y823) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the KIT antibody detected the KIT protein (arrow).)

Related Product Information for anti-KIT antibody

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-KIT antibody

Antibodies; Protein Kinases

References

Molderings, G.J., et al. Immunogenetics 62 (11-12), 721-727 (2010): Cheng, M., et al. Circ. Res. 107(9):1083-1093(2010). Chi, P., et al. Nature 467(7317):849-853(2010). Rossi, S., et al. Am. J. Surg. Pathol. 34(10):1480-1491(2010). Chen, P., et al. World J. Gastroenterol. 16(33):4227-4232(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4557695
• NCBI GeneID: 3815
• NCBI Accession #: NP_000213.1
• NCBI GenBank Nucleotide #: NM_000222.2
• UniProt Accession #: P05532; Q6IQ28; Q99662; Q9UM99; B5A956; D5LXN2; D5M931; F5H8F8; P10721
• Molecular Weight: 109,865 Da
• NCBI Official Full Name: mast/stem cell growth factor receptor Kit isoform 1
• NCBI Official Synonym Full Names: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• NCBI Official Symbol: KIT
• NCBI Official Synonym Symbols: PBT; SCFR; C-Kit; CD117
• NCBI Protein Information: mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like
• UniProt Protein Name: Mast/stem cell growth factor receptor Kit
• Protein Family: Kit ligand
• UniProt Gene Name: Kit
• UniProt Synonym Gene Names: Sl; SCFR

NCBI Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 5 C3.3|5 39.55 cM

Cellular Component: acrosome; cell surface; cytoplasm; external side of plasma membrane; extracellular space; intercellular junction; internal side of plasma membrane

Molecular Function: cytokine binding; protease binding; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; cell differentiation; chemotaxis; cytokine and chemokine mediated signaling pathway; dendritic cell cytokine production; detection of mechanical stimulus involved in sensory perception of sound; embryonic hemopoiesis; epithelial cell proliferation; erythrocyte differentiation; germ cell development; germ cell migration; germ cell programmed cell death; glycosphingolipid metabolic process; gut development; hemopoiesis; immature B cell differentiation; inflammatory response; lamellipodium biogenesis; lymphoid progenitor cell differentiation; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; melanocyte differentiation; myeloid leukocyte differentiation; myeloid progenitor cell differentiation; negative regulation of programmed cell death; ovarian follicle development; peptidyl-tyrosine phosphorylation; pigmentation; pigmentation during development; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of long-term neuronal synaptic plasticity; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of Notch signaling pathway; positive regulation of pseudopodium formation; positive regulation of transcription factor activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell shape; regulation of pigmentation during development; response to radiation; somatic stem cell division; somatic stem cell maintenance; spermatid development; spermatogenesis; stem cell differentiation; T cell differentiation; visual learning