Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Mindbomb Homolog 2 (MIB2) Polyclonal Antibody | anti-MIB2 antibody
Polyclonal Antibody to Mindbomb Homolog 2 (MIB2)
Gene Names
MIB2; ZZZ5; ZZANK1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Mindbomb Homolog 2 (MIB2); Polyclonal Antibody; Polyclonal Antibody to Mindbomb Homolog 2 (MIB2); anti-MIB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MIB2. It has been selected for its ability to recognize MIB2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGAS GIVEVLTEVP NIDVTATNSQ GFTLLHHASL KGHALAVRKI LARARQLVDA KKEDGFTALH LAALNNHREV AQILIREGRC DVNVRNRKLQ SPLHLAVQQA HVGLVPLLVD AGCSVNAEDE EGDTALHVAL QRHQLLPLVA DGAGGDPGPL QLLSRLQASG LPGSAELTVG AAVACFLALE GADVSYTNHR GRSPLDLAAE GRVLKALQGC AQRFRERQAG GGAAPGPRQT LGTPNTVTNL
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGAS GIVEVLTEVP NIDVTATNSQ GFTLLHHASL KGHALAVRKI LARARQLVDA KKEDGFTALH LAALNNHREV AQILIREGRC DVNVRNRKLQ SPLHLAVQQA HVGLVPLLVD AGCSVNAEDE EGDTALHVAL QRHQLLPLVA DGAGGDPGPL QLLSRLQASG LPGSAELTVG AAVACFLALE GADVSYTNHR GRSPLDLAAE GRVLKALQGC AQRFRERQAG GGAAPGPRQT LGTPNTVTNL
Sequence Length
922
Applicable Applications for anti-MIB2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MIB2 (Thr687~Leu930) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083221
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
115,861 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase MIB2 isoform 2
NCBI Official Synonym Full Names
mindbomb E3 ubiquitin protein ligase 2
NCBI Official Symbol
MIB2
NCBI Official Synonym Symbols
ZZZ5; ZZANK1
NCBI Protein Information
E3 ubiquitin-protein ligase MIB2
UniProt Protein Name
E3 ubiquitin-protein ligase MIB2
Protein Family
UniProt Gene Name
MIB2
UniProt Synonym Gene Names
SKD; ZZANK1; Novelzin
NCBI Description
The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017]
Uniprot Description
MIB2: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. 9 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: cytoplasm; cytosol; early endosome; ubiquitin ligase complex
Molecular Function: actin binding; protein binding; signal transducer activity; ubiquitin-protein transferase activity; zinc ion binding
Biological Process: Notch signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; protein polyubiquitination
Research Articles on MIB2
Similar Products
Product Notes
The MIB2 mib2 (Catalog #AAA2005279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mindbomb Homolog 2 (MIB2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mindbomb Homolog 2 (MIB2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MIB2 mib2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TGAS GIVEVLTEVP NIDVTATNSQ GFTLLHHASL KGHALAVRKI LARARQLVDA KKEDGFTALH LAALNNHREV AQILIREGRC DVNVRNRKLQ SPLHLAVQQA HVGLVPLLVD AGCSVNAEDE EGDTALHVAL QRHQLLPLVA DGAGGDPGPL QLLSRLQASG LPGSAELTVG AAVACFLALE GADVSYTNHR GRSPLDLAAE GRVLKALQGC AQRFRERQAG GGAAPGPRQT LGTPNTVTNL. It is sometimes possible for the material contained within the vial of "Mindbomb Homolog 2 (MIB2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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