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Western Blot (WB) (MID2 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MID2 antibody detected the MID2 protein (arrow).)

Rabbit MID2 Polyclonal Antibody | anti-MID2 antibody

MID2 Antibody (C-term)

Gene Names
MID2; FXY2; RNF60; TRIM1; MRX101
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MID2; Polyclonal Antibody; MID2 Antibody (C-term); Probable E3 ubiquitin-protein ligase MID2; 632-; Midin-2; Midline defect 2; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1; FXY2; RNF60; TRIM1; anti-MID2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MID2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-717 amino acids from the C-terminal region of human MID2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
690-717
Sequence Length
735
Applicable Applications for anti-MID2 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(MID2 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MID2 antibody detected the MID2 protein (arrow).)

Western Blot (WB) (MID2 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MID2 antibody detected the MID2 protein (arrow).)

Immunohistochemistry (IHC)

(MID2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MID2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (MID2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MID2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-MID2 antibody
The protein encoded by this gene is a member of the
tripartite motif (TRIM) family. The TRIM motif includes three
zinc-binding domains, a RING, a B-box type 1 and a B-box type 2,
and a coiled-coil region. The protein localizes to microtubular
structures in the cytoplasm. Alternate splicing of this gene
results in two transcript variants encoding different isoforms.
Product Categories/Family for anti-MID2 antibody
References
Lim, J., et al. Cell 125(4):801-814(2006)
Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005)
Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004)
Short, K.M., et al. BMC Cell Biol. 3, 1 (2002) :
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83210
NCBI Official Full Name
probable E3 ubiquitin-protein ligase MID2 isoform 1
NCBI Official Synonym Full Names
midline 2
NCBI Official Symbol
MID2
NCBI Official Synonym Symbols
FXY2; RNF60; TRIM1; MRX101
NCBI Protein Information
probable E3 ubiquitin-protein ligase MID2
UniProt Protein Name
Probable E3 ubiquitin-protein ligase MID2
UniProt Gene Name
MID2
UniProt Synonym Gene Names
FXY2; RNF60; TRIM1
UniProt Entry Name
TRIM1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Uniprot Description

MID2: is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009]

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Microtubule-binding

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: microtubule; cytoplasm

Molecular Function: protein homodimerization activity; zinc ion binding; protein heterodimerization activity; microtubule binding; phosphoprotein binding; ligase activity

Biological Process: negative regulation of viral transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of virion penetration into host cell; innate immune response; protein ubiquitination; positive regulation of transcription factor activity; activation of NF-kappaB transcription factor

Disease: Mental Retardation, X-linked 101

Research Articles on MID2

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Product Notes

The MID2 mid2 (Catalog #AAA9205153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 690-717. The MID2 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MID2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the MID2 mid2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MID2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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