Western Blot (WB)
(Western blot analysis of MIC1 using HeLa whole cell lysates)
Rabbit anti-Human, Mouse MIC1 Polyclonal Antibody | anti-MIC1 antibody
MIC1 Antibody
Gene Names
RMC1; MIC1; Mic-1; WDR98; C18orf8; HsT2591
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MIC1; Polyclonal Antibody; MIC1 Antibody; C18orf8; Chromosome 18 open reading frame 8; Colon cancer associated protein Mic1; Colon cancer-associated protein Mic1; HsT2591; Mic-1; MIC1_HUMAN; Uncharacterized protein C18orf8; anti-MIC1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
MIC1 antibody detects endogenous levels of total MIC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
500
Applicable Applications for anti-MIC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human MIC1
Subcellular Location
Endosome; Lysosome
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of MIC1 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of MIC1 using HeLa whole cell lysates)
Immunofluorescene (IF)
(MBS9608994 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9608994 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-MIC1 antibody
Function: Componement of the CCZ1-MON1 RAB7A guanine exchange factor (GEF). Acts as a positive regulator of CCZ1-MON1A/B function necessary for endosomal/autophagic flux and efficient RAB7A localization (PubMed:29038162).
Subunit Structure: Found in a complex with RMC1, CCZ1 MON1A and MON1B.
Similarity: Belongs to the RMC1 family.
Subunit Structure: Found in a complex with RMC1, CCZ1 MON1A and MON1B.
Similarity: Belongs to the RMC1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 74 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
regulator of MON1-CCZ1 complex isoform 3
NCBI Official Synonym Full Names
regulator of MON1-CCZ1
NCBI Official Symbol
RMC1
NCBI Official Synonym Symbols
MIC1; Mic-1; WDR98; C18orf8; HsT2591
NCBI Protein Information
regulator of MON1-CCZ1 complex
UniProt Protein Name
Regulator of MON1-CCZ1 complex
Protein Family
UniProt Gene Name
RMC1
UniProt Synonym Gene Names
Mic-1
NCBI Description
This gene encodes a colon cancer associated protein. [provided by RefSeq, Jan 2013]
Uniprot Description
Componement of the CCZ1-MON1 RAB7A guanine exchange factor (GEF). Acts as a positive regulator of CCZ1-MON1A/B function necessary for endosomal/autophagic flux and efficient RAB7A localization (PubMed:29038162).
Similar Products
Product Notes
The MIC1 rmc1 (Catalog #AAA9608994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIC1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MIC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MIC1 rmc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
