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Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5) antibody at 1:200 dilution.)

Rabbit Ku80 Polyclonal Antibody | anti-Ku80 antibody

Ku80 Antibody

Gene Names
Ku80; BG:DS09217.6; CG18801; DmelCG18801; DmKu80; Ku; ku80; KU80
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
Ku80; Polyclonal Antibody; Ku80 Antibody; Affinity Purified Rabbit Polyclonal Ku80 Antibody; Polyclonal Ku80 antibody; Anti-Ku80 antibody; KARP1 antibody; FLJ39089 antibody; KUB2 antibody; NFIV antibody; Ku-80; Ku86 autoantigen related protein 1 antibody; ATP-dependent DNA helicase II 80 kDa subunit antibody; Ku 80 antibody; Ku-80 antibody; Ku 80; KU80 antibody; KARP-1 antibody; XRCC5 antibody; TLAA antibody; Ku86 antibody; CTC85 antibody; anti-Ku80 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1.01 mg/ml (varies by lot)
Sequence Length
699
Applicable Applications for anti-Ku80 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:200
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5) antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5) antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using Ku80 antibody at a dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded Cal27 Xenograft using Ku80 antibody at a dilution of 1:100)

Western Blot (WB)

(Western blot analysis of 30 ug whole cell lysate (A:MOLT4) using a 7.5% SDS PAGE gel and Ku80 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug whole cell lysate (A:MOLT4) using a 7.5% SDS PAGE gel and Ku80 antibody at a dilution of 1:1000)
Related Product Information for anti-Ku80 antibody
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of VJ recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo VJ recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Product Categories/Family for anti-Ku80 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83 kDa
NCBI Official Full Name
Ku80
NCBI Official Symbol
Ku80
NCBI Official Synonym Symbols
BG:DS09217.6; CG18801; DmelCG18801; DmKu80; Ku; ku80; KU80
NCBI Protein Information
Ku80

Research Articles on Ku80

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Product Notes

The Ku80 (Catalog #AAA5312284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ku80 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:200 ICC: 1:100-1:200 IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the Ku80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ku80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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