Rabbit MGP Polyclonal Antibody | anti-MGP antibody

MGP Polyclonal Antibody

Cat. Num. AAA3011286

• Gene Names: MGP; NTI; GIG36; MGLAP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MGP; Polyclonal Antibody; MGP Polyclonal Antibody; Cell growth inhibiting gene 36 protein; Cell growth-inhibiting gene 36 protein; GAMMA-CARBOXYGLUTAMIC ACID PROTEIN; MATRIX; GIG36; Matrix Gla protein; MGLAP; MGP_HUMAN; NTI; anti-MGP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MGP polyclonal antibody detects endogenous levels of MGP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 103

• Applicable Applications for anti-MGP antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant full length Human MGP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MGP pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:PC3 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using MGP antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using MGP antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-MGP antibody

Matrix Gla protein, or MGP, is involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein containing five to six residues of g-carboxy-glutamic acid (Gla), a Ca2+ binding amino acid requiring vitamin K-dependent g carboxylase for its formation. In humans MGP is an 84 residue protein along with a 19 amino acid transmembrane signal peptide. A shortened 77 residue form of MGP is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity. High levels of ex-pression occur in heart, kidney and lung, and over-expression of MGP occurs in the breast cancer cell line 600 PEI. Retinoic acid induces MGP expression in chondrocytes, fibroblasts and osteoblasts. Mutations in the gene coding for MGP can cause Keutel syndrome (KS), associated with abnormal cartilage calcification, substantiating the role of MGP in extracellular matrix calcification regulation. MGP can bind Vitronectin and Fibronectin via its C-terminus; phosphorlyation of MGP occurs near the N-terminus at three serine residues, which are part of a tandemly repeated Ser-X-Glu sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 187591
• NCBI GeneID: 4256
• UniProt Accession #: P08493
• Molecular Weight: ~ 12kDa
• NCBI Official Full Name: matrix Gla protein
• NCBI Official Synonym Full Names: matrix Gla protein
• NCBI Official Symbol: MGP
• NCBI Official Synonym Symbols: NTI; GIG36; MGLAP
• NCBI Protein Information: matrix Gla protein
• UniProt Protein Name: Matrix Gla protein
• Protein Family: Matrix Gla protein
• UniProt Gene Name: MGP
• UniProt Synonym Gene Names: MGLAP; MGP
• UniProt Entry Name: MGP_HUMAN

NCBI Description

This gene encodes a member of the osteocalcin/matrix Gla family of proteins. The encoded vitamin K-dependent protein is secreted by chondrocytes and vascular smooth muscle cells, and functions as a physiological inhibitor of ectopic tissue calcification. Carboxylation status of the encoded protein is associated with calcification of the vasculature in human patients with cardiovascular disease and calcification of the synovial membranes in osteoarthritis patients. Mutations in this gene cause Keutel syndrome in human patients, which is characterized by abnormal cartilage calcification, peripheral pulmonary stenosis and facial hypoplasia. [provided by RefSeq, Sep 2016]

Uniprot Description

MGP: Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation. Defects in MGP are the cause of Keutel syndrome (KS). KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Belongs to the osteocalcin/matrix Gla protein family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: protein binding; structural constituent of bone; extracellular matrix structural constituent; calcium ion binding

Biological Process: ossification; cell differentiation; regulation of bone mineralization; cartilage condensation

Disease: Keutel Syndrome