Rabbit anti-Rat mGluR1a Polyclonal Antibody | anti-GRM1 antibody
mGluR1a (Metabotropic Glutamate Receptor)
Purified by Immunoaffinity chromatography.
Purified by Immunoaffinity chromatography.
Dilution: Western Blot: 0.25-1ug/ml detects a single band at ~140kD.
Immunocytochemistry: 0.5-2ug/ml using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment.
Storage and Stability:
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a metabotropic glutamate receptor that functions by activating phospholipase C. L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The canonical alpha isoform of the encoded protein is a disulfide-linked homodimer whose activity is mediated by a G-protein-coupled phosphatidylinositol-calcium second messenger system. This gene may be associated with many disease states, including schizophrenia, bipolar disorder, depression, and breast cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
Uniprot Description
mGluR1: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum. Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP. Belongs to the G-protein coupled receptor 3 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 3
Chromosomal Location of Human Ortholog: 6q24
Cellular Component: integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; nucleus
Molecular Function: G-protein coupled receptor activity; protein binding; glutamate receptor activity
Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; activation of MAPK activity; protein kinase C activation; metabotropic glutamate receptor signaling pathway; locomotory behavior; regulation of sensory perception of pain; sensory perception of pain
Disease: Spinocerebellar Ataxia, Autosomal Recessive 13
Research Articles on GRM1
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Product Notes
The GRM1 grm1 (Catalog #AAA613086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mGluR1a (Metabotropic Glutamate Receptor) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's mGluR1a can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 0.25-1ug/ml detects a single band at ~140kD. Immunocytochemistry: 0.5-2ug/ml using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Storage and Stability: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Researchers should empirically determine the suitability of the GRM1 grm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mGluR1a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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