Rabbit TSTA3 / FX Polyclonal Antibody | anti-TSTA3 antibody

Rabbit Polyclonal (IgG) to Human TSTA3 / FX

Cat. Num. AAA248011

• Gene Names: TSTA3; FX; P35B; SDR4E1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TSTA3 / FX; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TSTA3 / FX; Anti-TSTA3 / FX Antibody (aa221-270) IHC-plus; TSTA3; 3-5 epimerase/4-reductase; FX; GDP-L-fucose synthase; SDR4E1; p35B; Protein FX; Human TSTA3; anti-TSTA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TSTA3 Antibody detects endogenous levels of total TSTA3 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 321

• Applicable Applications for anti-TSTA3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TSTA3 (aa221-270).
• Immunogen: TSTA3 / FX antibody was raised against synthetic peptide from human TSTA3 (aa221-270).
• Antigen Modification: aa221-270
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TSTA3 / FX antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from rat kidney/rat muscle cells, using TSTA3 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507709
• NCBI GeneID: 7264
• NCBI Accession #: NP_003304.1
• NCBI GenBank Nucleotide #: NM_003313.3
• UniProt Accession #: Q13630; Q567Q9; Q9UDG7; B2R8Y7; D3DWK5
• Molecular Weight: 35,893 Da
• NCBI Official Full Name: GDP-L-fucose synthase
• NCBI Official Synonym Full Names: tissue specific transplantation antigen P35B
• NCBI Official Symbol: TSTA3
• NCBI Official Synonym Symbols: FX; P35B; SDR4E1
• NCBI Protein Information: GDP-L-fucose synthase; 3-5 epimerase/4-reductase; red cell NADP(H)-binding protein; tissue specific transplantation antigen 3; GDP-4-keto-6-deoxy-D-mannose epimerase-reductase; GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase; short chain dehydrogenase/reductase family 4E, member 1
• UniProt Protein Name: GDP-L-fucose synthase
• UniProt Gene Name: TSTA3
• UniProt Synonym Gene Names: SDR4E1
• UniProt Entry Name: FCL_HUMAN

NCBI Description

Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. [provided by RefSeq, Jul 2008]

Uniprot Description

TSTA3: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. Belongs to the fucose synthase family.

Protein type: Oxidoreductase; EC 1.1.1.271; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm

Molecular Function: GDP-L-fucose synthase activity; electron carrier activity; isomerase activity; coenzyme binding; GDP-4-dehydro-D-rhamnose reductase activity

Biological Process: leukocyte adhesion; cytolysis; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process