Human Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit | PTGS1 elisa kit

Human Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit

Cat. Num. AAA2012484

• Gene Names: PTGS1; COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
• Reactivity: Human
• Synonyms: Prostaglandin Endoperoxide Synthase 1 (PTGS1); Human Prostaglandin Endoperoxide Synthase 1 (PTGS1) ELISA Kit; COX1; COX3; PCOX1; PGG/HS; PGHS1; PHS1; PTGHS; Cyclooxygenase 1; Prostaglandin H2 Synthase 1; Prostaglandin G/H Synthase And Cyclooxygenase; PTGS1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 1 (PTGS1).; No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 1 (PTGS1) and analogues was observed.
• Sequence Length: 599

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.225ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 1 (PTGS1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 1 (PTGS1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTGS1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 1 (PTGS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 1 (PTGS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 1 (PTGS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 1 (PTGS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PTGS1 elisa kit

Signal transduction; Enzyme & Kinase; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 17017287
• NCBI GeneID: 5742
• UniProt Accession #: P23219; Q15122; Q3HY28; Q3HY29; Q5T7T6; Q5T7T7; Q5T7T8; A8K1V7; B4DHQ2; B4E2S5
• Molecular Weight: 72,010 Da
• NCBI Official Full Name: prostaglandin-endoperoxide synthase 1
• NCBI Official Synonym Full Names: prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
• NCBI Official Symbol: PTGS1
• NCBI Official Synonym Symbols: COX1; COX3; PHS1; PCOX1; PES-1; PGHS1; PTGHS; PGG/HS; PGHS-1
• NCBI Protein Information: prostaglandin G/H synthase 1
• UniProt Protein Name: Prostaglandin G/H synthase 1
• Protein Family: Prostaglandin G/H synthase
• UniProt Gene Name: PTGS1
• UniProt Synonym Gene Names: COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1
• UniProt Entry Name: PGH1_HUMAN

NCBI Description

This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

COX-1: May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. Homodimer. Belongs to the prostaglandin G/H synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.14.99.1

Chromosomal Location of Human Ortholog: 9q32-q33.3

Cellular Component: endoplasmic reticulum membrane; photoreceptor outer segment; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: prostaglandin-endoperoxide synthase activity; peroxidase activity; dioxygenase activity; metal ion binding; heme binding

Biological Process: xenobiotic metabolic process; regulation of blood pressure; cyclooxygenase pathway; arachidonic acid metabolic process; lipid metabolic process; response to oxidative stress; inflammatory response; prostaglandin biosynthetic process; regulation of cell proliferation