Rabbit Metabotropic Glutamate Receptor 7 Polyclonal Antibody | anti-GRM7 antibody

Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody (C-term)

Cat. Num. AAA9217091

• Gene Names: GRM7; GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: Metabotropic Glutamate Receptor 7; Polyclonal Antibody; Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody (C-term); Metabotropic glutamate receptor 7; mGluR7; GRM7; GPRC1G; MGLUR7; anti-GRM7 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Metabotropic Glutamate Receptor 7 (GPRC1G) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 857-887 amino acids from the C-terminal region of human Metabotropic Glutamate Receptor 7 (GPRC1G).
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 922

• Applicable Applications for anti-GRM7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB:~~1:1000; IHC-P:~~1:10~50
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Cell membrane; Multi-pass membrane protein.
• Tissue Location: Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.
• Antigen Region: 857-887 aa
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-GPRC1G Antibody (C-term) at 1:1000 dilution + SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GRM7 antibody

Description: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of GRM8 have been described but their full-length nature has not been determined.

Background: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity.

Product Categories/Family for anti-GRM7 antibody

Primary Antibodies; Antibody Collections; Neuroscience; GPCR Antibodies

References

Schulz, H.L., et al., Neurosci. Lett. 326 (1):37-40 (2002). Flor, P.J., et al., Neuropharmacology 36 (2):153-159 (1997). Makoff, A., et al., Brain Res. Mol. Brain Res. 40 (1):165-170 (1996). Scherer, S.W., et al., Genomics 31 (2):230-233 (1996). Okamoto, N., et al., J. Biol. Chem. 269 (2):1231-1236 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 32528268
• NCBI GeneID: 2917
• NCBI Accession #: NP_870989
• NCBI GenBank Nucleotide #: NM_181874.2
• UniProt Accession #: Q14831; Q8NFS2; Q8NFS3; Q8NFS4
• Molecular Weight: 102251 Da
• NCBI Official Full Name: metabotropic glutamate receptor 7 isoform b
• NCBI Official Synonym Full Names: glutamate metabotropic receptor 7
• NCBI Official Symbol: GRM7
• NCBI Official Synonym Symbols: GLUR7; MGLU7; GPRC1G; MGLUR7; PPP1R87
• NCBI Protein Information: metabotropic glutamate receptor 7
• UniProt Protein Name: Metabotropic glutamate receptor 7
• Protein Family: Metabotropic glutamate receptor
• UniProt Gene Name: GRM7
• UniProt Synonym Gene Names: GPRC1G; MGLUR7; mGluR7
• UniProt Entry Name: GRM7_HUMAN

NCBI Description

L-glutamate is the major excitatory neurotransmitter in the central nervous system, and it activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors that have been divided into three groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5, and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3, while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

mGluR7: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. Belongs to the G-protein coupled receptor 3 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 3; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 3p26.1-p25.1

Cellular Component: asymmetric synapse; axon; cell cortex; dendrite; dendritic shaft; integral to membrane; integral to plasma membrane; plasma membrane; postsynaptic membrane; presynaptic active zone; presynaptic membrane; receptor complex

Molecular Function: calcium channel regulator activity; calcium ion binding; glutamate binding; glutamate receptor activity; group III metabotropic glutamate receptor activity; PDZ domain binding; voltage-gated calcium channel activity

Biological Process: adult behavior; behavioral fear response; conditioned taste aversion; metabotropic glutamate receptor, adenylate cyclase inhibiting pathway; negative regulation of adenylate cyclase activity; negative regulation of cAMP biosynthetic process; negative regulation of glutamate secretion; regulation of cyclase activity; regulation of synaptic transmission, glutamatergic; sensory perception of smell; sensory perception of sound; short-term memory; synaptic transmission; transmission of nerve impulse