Rabbit MERTK/TYRO3 Polyclonal Antibody | anti-MERTK antibody

MERTK/TYRO3 polyclonal antibody

Cat. Num. AAA3007732

• Gene Names: MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MERTK/TYRO3; Polyclonal Antibody; MERTK/TYRO3 polyclonal antibody; Tyrosine-protein kinase Mer; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; MERTK; MER; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase BYK; Tyrosine-protein kinase DTK; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY; Tyrosine-protein kinase TIF; TYRO3; BYK; DTK; RSE; SKY; TIF; anti-MERTK antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: MERTK/TYRO3 polyclonal antibody detects endogenous levels of MERTK/TYRO3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
• Sequence Length: 999

• Applicable Applications for anti-MERTK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human MERTK/TYRO3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MERTK/TYRO3 polyclonal antibody at 1:500 dilutionLane1:A375 whole cell lysate (20ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:The Brain tissue lysate of Rat (40ug))

Related Product Information for anti-MERTK antibody

Receptor tyrosine kinases (RTKs) represent an important class of transmembrane signaling molecules. Binding of the extracellular domain of an RTK to its cognate ligand leads to receptor dimerization and the activation of the intrinsic tyrosine kinase activity of its intracellular kinase domain. The Axl/ UFO subfamily of receptor tyrosine kinases is comprised of members Tyro3 (also referred to as BYK, Brt, Dtk, RSE, Tif or Sky), Axl (also called Tyro7 or UFO) and Mer (also called Nyk, c-Eyk and Tyro12). Members of this family have a common molecular structure which contains an N-terminal extracellular domain comprised of two Ig domains, two FNIII domains and a membrane spanning single helix followed by the cytoplasmic tyrosine kinase domain. These RTKs are functionally significant in spermatogenesis, immunoregulation and phagocytosis. Tyro3, Axl and Mer are widely expressed in adult tissues with their expression most abundant in brain, testis, lymphatic and vascular tissue. Tyro3 has been shown to undergo posttranslational modifications including both tyrosine phosphorylation as well as glycosylation. Two proteins, protein S and Gas6, have been proposed as ligands for the Axl/UFO family of receptors. Both function as anti-coagulants through an unknown mechanism. Gas6 was cloned as a growth arrest-specific gene, while protein S is an abundant serum protein which is thought to act by indirectly inhibiting proteases involved in the coagulation response.

NCBI and Uniprot Product Information

• NCBI GI #: 66932918
• NCBI GeneID: 10461
• NCBI Accession #: NP_006334.2
• NCBI GenBank Nucleotide #: NP_006334.2
• UniProt Accession #: Q12866; Q06418
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: tyrosine-protein kinase Mer
• NCBI Official Synonym Full Names: MER proto-oncogene, tyrosine kinase
• NCBI Official Symbol: MERTK
• NCBI Official Synonym Symbols: MER; RP38; c-Eyk; c-mer; Tyro12
• NCBI Protein Information: tyrosine-protein kinase Mer
• UniProt Protein Name: Tyrosine-protein kinase Mer
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: MERTK
• UniProt Synonym Gene Names: MER
• UniProt Entry Name: MERTK_HUMAN

NCBI Description

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]

Uniprot Description

Mer: a proto-oncogene receptor tyrosine-protein kinase of the Axl family. Binds several ligands including LGALS3, Tubby, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3

Protein type: Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Axl family

Chromosomal Location of Human Ortholog: 2q14.1

Cellular Component: extracellular space; photoreceptor outer segment; integral to plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: platelet activation; negative regulation of lymphocyte activation; peptidyl-tyrosine phosphorylation; vagina development; protein amino acid phosphorylation; phagocytosis; cell surface receptor linked signal transduction; cell-cell signaling; natural killer cell differentiation; retina development in camera-type eye; positive regulation of phagocytosis; protein kinase B signaling cascade; spermatogenesis; blood coagulation; leukocyte migration; apoptotic cell clearance; secretion by cell

Disease: Retinitis Pigmentosa 38