Western Blot (WB)
(Western Blot analysis of Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) (with 5ng, 2ng and 1ng) using Anti-MERS-CoV Spike Polyclonal Antibody at dilution of 1:1000.)
Rabbit MERS-CoV Spike Polyclonal Antibody | anti-MERS-CoV antibody
Anti-MERS-CoV Spike Polyclonal Antibody
Reactivity
MERS-CoV
Applications
Western Blot, ELISA
Purity
Antigen Affinity
Synonyms
MERS-CoV Spike; Polyclonal Antibody; Anti-MERS-CoV Spike Polyclonal Antibody; coronavirus s1; coronavirus s2; coronavirus spike; cov spike; ncov RBD; ncov s1; ncov s2; ncov spike; RBD; S; s1; Spike RBD; anti-MERS-CoV antibody
Host
Rabbit
Reactivity
MERS-CoV
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity
Form/Format
0.2um filtered solution in PBS
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MERS-CoV antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa1-1297, His Tag)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) (with 5ng, 2ng and 1ng) using Anti-MERS-CoV Spike Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Recombinant MERS-CoV Spike Protein (S1+S2 ECD, aa 1-1297, His Tag) (with 5ng, 2ng and 1ng) using Anti-MERS-CoV Spike Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for anti-MERS-CoV antibody
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
Product Categories/Family for anti-MERS-CoV antibody
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Product Notes
The MERS-CoV (Catalog #AAA2563985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MERS-CoV Spike Polyclonal Antibody reacts with MERS-CoV and may cross-react with other species as described in the data sheet. AAA Biotech's MERS-CoV Spike can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-1:5000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the MERS-CoV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MERS-CoV Spike, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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