Western Blot (WB)
(Western blot analysis of extracts from 293 cells(UV treatment), using LRRK2 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human LRRK2 Polyclonal Antibody | anti-LRRK2 antibody
LRRK2 Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
LRRK2; Polyclonal Antibody; LRRK2 Antibody; augmented in rheumatoid arthritis 17; AURA17; Dardarin; Leucine rich repeat kinase 2; leucine rich repeat serine threonine protein kinase 2; Leucine-rich repeat serine/threonine-protein kinase 2; LRRK 2; LRRK2_HUMAN; PARK 8; PARK8; RIPK7; ROCO 2; ROCO2; anti-LRRK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
LRRK2 Antibody detects endogenous levels of total LRRK2.
Tissue Specificity: Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed in neutrophils (at protein level). Expressed in the brain. Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
Tissue Specificity: Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed in neutrophils (at protein level). Expressed in the brain. Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LRRK2 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human LRRK2.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylated. Phosphorylation of Ser-910 and either Ser-935 or Ser-1444 facilitates interaction with YWHAG. Phosphorylation of Ser-910 and/or Ser-935 facilitates interaction with SFN.
Subunit Structure
Homodimer. Interacts with PRKN, PRDX3, and TPCN2. Interacts with VPS35 and RAB29. Interacts (via ROC domain) with SEC16A. Interacts with APP; interaction promotes phosphorylation of 'Thr-743' of APP. Interacts with MAPT. Interacts with RAB8A, RAB10, and RAB12. Interacts with YWHAG; this interaction is dependent on phosphorylation of Ser-910 and either Ser-935 or Ser-1444. Interacts with SFN; this interaction is dependent on phosphorylation of Ser-910 and/or Ser-935.
Similarity
The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins (PubMed:24687852). It also mediates homodimerization and regulates kinase activity (PubMed:30635421).The Roc domain mediates homodimerization and regulates kinase activity.Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Subcellular Location
Cytoplasmic vesicle. Perikaryon. Golgi apparatus membrane>Peripheral membrane protein. Cell projection>Axon. Cell projection>Dendrite. Endoplasmic reticulum membrane>Peripheral membrane protein. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane>Peripheral membrane protein.
Note: Colocalized with RAB29 along tubular structures emerging from Golgi apparatus (PubMed:23395371). Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (PubMed:25201882).
Note: Colocalized with RAB29 along tubular structures emerging from Golgi apparatus (PubMed:23395371). Localizes to endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (PubMed:25201882).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from 293 cells(UV treatment), using LRRK2 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells(UV treatment), using LRRK2 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-LRRK2 antibody
Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. Regulates primary ciliogenesis through phosphorylation of RAB8A and RAB10, which promotes SHH signaling in the brain. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose-6-phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Plays an important role in recruiting SEC16A to endoplasmic reticulum exit sites (ERES) and in regulating ER to Golgi vesicle-mediated transport and ERES organization. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Phosphorylates PRDX3. By phosphorylating APP on 'Thr-743', which promotes the production and the nuclear translocation of the APP intracellular domain (AICD), regulates dopaminergic neuron apoptosis. Independent of its kinase activity, inhibits the proteosomal degradation of MAPT, thus promoting MAPT oligomerization and secretion. In addition, has GTPase activity via its Roc domain which regulates LRRK2 kinase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
2527
NCBI Official Full Name
leucine-rich repeat serine/threonine-protein kinase 2
NCBI Official Synonym Full Names
leucine-rich repeat kinase 2
NCBI Official Symbol
LRRK2
NCBI Official Synonym Symbols
PARK8; RIPK7; ROCO2; AURA17; DARDARIN
NCBI Protein Information
leucine-rich repeat serine/threonine-protein kinase 2; augmented in rheumatoid arthritis 17
UniProt Protein Name
Leucine-rich repeat serine/threonine-protein kinase 2
Protein Family
UniProt Gene Name
LRRK2
UniProt Synonym Gene Names
PARK8
UniProt Entry Name
LRRK2_HUMAN
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Product Notes
The LRRK2 lrrk2 (Catalog #AAA9613908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRRK2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LRRK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the LRRK2 lrrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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