Rabbit MERIT40 Polyclonal Antibody | anti-MERIT40 antibody

MERIT40 Antibody

Cat. Num. AAA9613505

• Gene Names: BABAM1; NBA1; HSPC142; MERIT40; C19orf62
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: MERIT40; Polyclonal Antibody; MERIT40 Antibody; BABA1_HUMAN; babam1; BRCA1 A complex subunit MERIT40; BRISC and BRCA1-A complex member 1; C19orf62; FLJ20571; HSPC142; hypothetical protein LOC29086; Mediator of Rap80 interactions and targeting 40kDa; Mediator of RAP80 interactions and targeting subunit of 40kDa; MERIT 40; NBA1; New component of the BRCA1 A complex; New component of the BRCA1-A complex; Uncharacterized protein C19orf62; anti-MERIT40 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MERIT40 Antibody detects endogenous levels of total MERIT40.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MERIT40 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human MERIT40, corresponding to a region within N-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Subunit Structure: Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1 and BABAM2. Component of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Identified in a complex with SHMT2 and the other subunits of the BRISC complex.
• Similarity: The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.Belongs to the BABAM1 family.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A-431  , using MERIT40 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MERIT40 antibody

Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 75709194
• NCBI GeneID: 29086
• NCBI Accession #: NP_001028721.1
• NCBI GenBank Nucleotide #: NM_001033549.2
• UniProt Accession #: Q9NWV8; Q6FIA0; Q9P018; A8MQT0; B4DRY9; B4DVR1
• Molecular Weight: 18,621 Da
• NCBI Official Full Name: BRISC and BRCA1-A complex member 1 isoform 1
• NCBI Official Synonym Full Names: BRISC and BRCA1 A complex member 1
• NCBI Official Symbol: BABAM1
• NCBI Official Synonym Symbols: NBA1; HSPC142; MERIT40; C19orf62
• NCBI Protein Information: BRISC and BRCA1-A complex member 1
• UniProt Protein Name: BRISC and BRCA1-A complex member 1
• UniProt Gene Name: BABAM1
• UniProt Entry Name: BABA1_HUMAN

Uniprot Description

NBA1: a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes. Two alternatively spliced human isoforms have been reported.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: double-strand break repair; double-strand break repair via nonhomologous end joining; G2/M transition DNA damage checkpoint; positive regulation of DNA repair; response to ionizing radiation