Rabbit MEPE Polyclonal Antibody | anti-MEPE antibody

MEPE Antibody

Cat. Num. AAA9630295

• Gene Names: MEPE; OF45
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MEPE; Polyclonal Antibody; MEPE Antibody; Matrix extracellular phosphoglycoprotein; MEPE_HUMAN; OF45; Osteoblast/osteocyte factor 45; anti-MEPE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MEPE Antibody detects endogenous levels of MEPE.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MEPE antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MEPE.
• Conjugation: Unconjugated
• Similarity: The acidic serine aspartate-rich MEPE-associated (ASARM) motif is sufficient when phosphorylated to inhibit bone mineralization by osteoblasts and cartilage mineralization by chondrocytes by binding hydroxyapatite crystals during the mineralization stage (PubMed:15664000, PubMed:18162525, PubMed:18597632, PubMed:19998030, PubMed:22766095). It can also inhibit dentin mineralization (PubMed:20581062). The dentonin region is sufficient to promote dental pulp stem cell proliferation (PubMed:15153459). It can also stimulate bone formation, osteoblast differentiation, and activate integrin signaling pathways (PubMed:15040834). Belongs to the PF07175/osteoregulin family.
• Post Translational Modifications: Phosphorylated on serine residues in the ASARM motif (in vitro) by FAM20C; the phosphorylation is important for the inhibition of bone mineralization.Cleaved by CTSB/cathepsin B; the cleavage is blocked by metalloprotease PHEX.
• Subunit Structure: Interacts (via the ASARM motif) with PHEX; the interaction is zinc-dependent.
• Tissue Specificity: Expressed by osteoblasts. Expressed by stem cells in dental pulp. Expressed by mesenchymal cells in dental papilla and dental pulp. Expressed in teeth, specifically in decidious dentin. Expressed in ondotoblasts. Expressed in salivary glands. Secreted from oncogenic hypophosphatemic tumors.
• Function: Promotes renal phosphate excretion and inhibits intestinal phosphate absorption. Promotes bone mineralization by osteoblasts and cartilage mineralization by chondrocytes. Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Promotes dental pulp stem cell proliferation and differentiation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-MEPE antibody

Secreted; Extracellular space; Extracellular matrix; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 296011082
• NCBI GeneID: 56955
• NCBI Accession #: NP_001171626.1
• NCBI GenBank Nucleotide #: NM_001184697.1
• UniProt Accession #: Q9NQ76; A1A4X9; A8MTA3; D2CFR4; F5H5C5
• Molecular Weight: 58,419 Da
• NCBI Official Full Name: matrix extracellular phosphoglycoprotein isoform b
• NCBI Official Synonym Full Names: matrix extracellular phosphoglycoprotein
• NCBI Official Symbol: MEPE
• NCBI Official Synonym Symbols: OF45
• NCBI Protein Information: matrix extracellular phosphoglycoprotein; osteoblast/osteocyte factor 45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone)
• UniProt Protein Name: Matrix extracellular phosphoglycoprotein
• Protein Family: Matrix extracellular phosphoglycoprotein
• UniProt Gene Name: MEPE
• UniProt Synonym Gene Names: OF45
• UniProt Entry Name: MEPE_HUMAN

NCBI Description

This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

MEPE: Promotes renal phosphate excretion and modulates mineralization. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: proteinaceous extracellular matrix

Molecular Function: protein binding; extracellular matrix structural constituent

Biological Process: negative regulation of bone mineralization; biomineral formation; regulation of bone remodeling; skeletal development