Western Blot (WB)
(Staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.)
Goat Melanophilin Polyclonal Antibody | anti-Mlph antibody
Goat anti-Melanophilin (mouse) Antibody
Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads.
Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. See also publication Figueiredo AC et al. , J Biol Chem.2008 Aug 22;283(34): 23209-16. (PMID: 18559336).
Western Blot (WB)
(Staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.)
Western Blot (WB)
(Staining (0.5ug/ml) of total melanocyte PNS (200ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may represent the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College.)
Immunofluorescence (IF)
(Wild-type C57bl6 derived melanocytes were stained (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.)
Immunofluorescence (IF)
(Wild-type C57bl6 derived melanocytes were stained (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.)
NCBI and Uniprot Product Information
Uniprot Description
MLPH: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3). GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Cellular Component: actin cytoskeleton; cortical actin cytoskeleton; dendrite; melanosome; microtubule organizing center; perinuclear region of cytoplasm; stress fiber
Molecular Function: actin binding; metal ion binding; microtubule plus-end binding; myosin binding; myosin V binding; protein binding; Rab GTPase binding
Biological Process: intracellular protein transport; melanocyte differentiation; melanosome localization; pigmentation; protein targeting
Research Articles on Mlph
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Product Notes
The Mlph mlph (Catalog #AAA421445) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Melanophilin (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Melanophilin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), GST-pull down. Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2ug/ml. Please note that this product may require overnight incubation of primary and high protein loads. Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes, see picture. See also publication Figueiredo AC et al., J Biol Chem.2008 Aug 22;283(34): 23209-16. (PMID: 18559336). Researchers should empirically determine the suitability of the Mlph mlph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ARHIFAKPVM AQQP. It is sometimes possible for the material contained within the vial of "Melanophilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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