Rabbit anti-Human MEK5 Polyclonal Antibody | anti-MEK5 antibody

MEK5 Polyclonal Antibody

Cat. Num. AAA3013026

• Gene Names: MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ProA affinity purified
• Synonyms: MEK5; Polyclonal Antibody; MEK5 Polyclonal Antibody; MAP kinase kinase 5; MAPKK 5; MEK 5; MAP2K5; Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5; PRKMK5; Protein kinase; mitogen-activated; kinase 5; SAPKK5; SKK5; anti-MEK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MEK5 polyclonal antibody detects endogenous levels of MEK5 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 412

• Applicable Applications for anti-MEK5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1,000-1:5,000; ICC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.)

Immunocytochemistry (ICC)

(ICC staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-MEK5 antibody

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 332164798
• NCBI GeneID: 5607
• NCBI Accession #: NP_001193733.1
• NCBI GenBank Nucleotide #: NP_001193733.1
• UniProt Accession #: Q13163
• Molecular Weight: 49kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 5 isoform C
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 5
• NCBI Official Symbol: MAP2K5
• NCBI Official Synonym Symbols: MEK5; MAPKK5; PRKMK5; HsT17454
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• UniProt Gene Name: MAP2K5
• UniProt Synonym Gene Names: MEK5; MKK5; PRKMK5; MAP kinase kinase 5; MAPKK 5; MEK 5

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq, May 2011]

Uniprot Description

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.