Rabbit anti-Mouse, Human MEF2B Polyclonal Antibody | anti-MEF2B antibody

Anti-MEF2B Antibody (aa51-100) IHC-plus

Cat. Num. AAA249718

• Gene Names: MEF2B; RSRFR2
• Reactivity: Mouse, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: MEF2B; Polyclonal Antibody; Anti-MEF2B Antibody (aa51-100) IHC-plus; Rabbit Polyclonal (IgG) to Human MEF2B; Human MEF2B; LOC729991-MEF2B readthrough; MEF2B neighbor gene protein; Myocyte enhancer factor 2B; LOC729991-MEF2B; MEF2BNB-MEF2B; MEF2BNB-MEF2B readthrough; anti-MEF2B antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MEF2B Antibody detects endogenous levels of total MEF2B protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 368

• Applicable Applications for anti-MEF2B antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000); IHC-P (10 ug/ml); WB (1:500 - 1:1000)
• Immunogen: MEF2B antibody was raised against synthetic peptide from human MEF2B (aa51-100).
• Immunogen Description: Synthetic peptide from human MEF2B (aa51-100).
• Antigen Modification: aa51-100
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-MEF2B antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HT-29 cells, using MEF2B Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 224809412
• NCBI GeneID: 100271849
• NCBI Accession #: NP_001139257.1
• NCBI GenBank Nucleotide #: NM_001145785.1
• Molecular Weight: 39,144 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2B
• NCBI Official Synonym Full Names: myocyte enhancer factor 2B
• NCBI Official Symbol: MEF2B
• NCBI Official Synonym Symbols: RSRFR2
• NCBI Protein Information: myocyte-specific enhancer factor 2B
• UniProt Protein Name: Myocyte-specific enhancer factor 2B
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2B
• UniProt Synonym Gene Names: XMEF2
• UniProt Entry Name: MEF2B_HUMAN

NCBI Description

The product of this gene is a member of the MADS/MEF2 family of DNA binding proteins. The protein is thought to regulate gene expression, including expression of the smooth muscle myosin heavy chain gene. This region undergoes considerable alternative splicing, with transcripts supporting two non-overlapping loci (GeneID 729991 and 100271849) as well as numerous read-through transcripts that span both loci (annotated as GeneID 4207). Several isoforms of this protein are expressed from either this locus or from some of the read-through transcripts annotated on GeneID 4207. [provided by RefSeq, Jan 2014]

Uniprot Description

MEF2B: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription. Belongs to the MEF2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleus; transcription factor complex

Molecular Function: histone deacetylase binding; protein dimerization activity; transcription factor activity

Biological Process: muscle development; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter