Rabbit anti-Human MED17 Polyclonal Antibody | anti-MED17 antibody

MED17, ID (MED17, ARC77, CRSP6, DRIP77, DRIP80, TRAP80, Mediator of RNA polymerase II transcription subunit 17, Activator-recruited cofactor 77kD component, Cofactor required for Sp1 transcriptional activation subunit 6, Mediator complex subunit 17, Thyro

Cat. Num. AAA6319961

• Gene Names: MED17; CRSP6; CRSP77; DRIP80; TRAP80
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MED17; Polyclonal Antibody; ID (MED17; ARC77; CRSP6; DRIP77; DRIP80; TRAP80; Mediator of RNA polymerase II transcription subunit 17; Activator-recruited cofactor 77kD component; Cofactor required for Sp1 transcriptional activation subunit 6; Mediator complex subunit 17; Thyro; Thyroid hormone receptor-associated protein complex 80kD component; Transcriptional coactivator CRSP77; Vitamin D3 receptor-interacting protein complex 80kD component; anti-MED17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MED17 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MED17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human MED17.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MED17 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MED17 antibody detected the MED17 protein (arrow).)

Related Product Information for anti-MED17 antibody

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Product Categories/Family for anti-MED17 antibody

Antibodies; Receptors

References

Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Jang, M.K., et al. Mol. Cell 19(4):523-534(2005). Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004). Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003). Lau, J.F., et al. Mol. Cell. Biol. 23(2):620-628(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 28558975
• NCBI GeneID: 9440
• NCBI Accession #: NP_004259.3
• NCBI GenBank Nucleotide #: NM_004268.4
• UniProt Accession #: Q9NVC6; Q9HA81; Q9UNP7; Q9Y2W0; Q9Y660; B3KN07
• Molecular Weight: 72,890 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 17
• NCBI Official Synonym Full Names: mediator complex subunit 17
• NCBI Official Symbol: MED17
• NCBI Official Synonym Symbols: CRSP6; CRSP77; DRIP80; TRAP80
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 17; ARC77; CRSP complex subunit 6; transcriptional coactivator CRSP77; activator-recruited cofactor 77 kDa component; vitamin D3 receptor-interacting protein complex 80 kDa component; thyroid hormone rec
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 17
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED17
• UniProt Synonym Gene Names: ARC77; CRSP6; DRIP77; DRIP80; TRAP80; ARC77; CRSP complex subunit 6; Trap80; DRIP80
• UniProt Entry Name: MED17_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]

Uniprot Description

MED17: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Defects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA). It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth. Belongs to the Mediator complex subunit 17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: nucleoplasm; transcription factor complex; membrane; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; stem cell maintenance; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter

Disease: Microcephaly, Postnatal Progressive, With Seizures And Brain Atrophy