Rabbit anti-Human, Mouse MED13 Polyclonal Antibody | anti-MED13 antibody

MED13 Antibody

Cat. Num. AAA9629362

• Gene Names: MED13; ARC250; THRAP1; DRIP250; HSPC221; TRAP240
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MED13; Polyclonal Antibody; MED13 Antibody; 1110067M05Rik; Activator-recruited cofactor 250 kDa component; ARC250; D030023K18; DRIP 250; DRIP250; HSPC221; KIAA0593; Mediator complex subunit 13; Mediator of RNA polymerase II transcription subunit 13 homolog; Mediator of RNA polymerase II transcription; subunit 13; OTTMUSP00000001175; RP23-50E4.3; THRAP1; Thyroid hormone receptor associated protein 240 kDa subunit; Thyroid hormone receptor associated protein complex component TRAP240; Thyroid hormone receptor-associated protein 1; Thyroid hormone receptor-associated protein complex 240 kDa component; Trap240; Vitamin D3 receptor-interacting protein complex component DRIP250; anti-MED13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MED13 Antibody detects endogenous levels of total MED13.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MED13 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the Mediator complex subunit 13 family.
• Subunit Structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
• Tissue Specificity: Ubiquitous.
• Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-MED13 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 102468717
• NCBI GeneID: 9969
• NCBI Accession #: NP_005112.2
• NCBI GenBank Nucleotide #: NM_005121.2
• UniProt Accession #: Q9UHV7; O60334; B2RU05
• Molecular Weight: 239,297 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 13
• NCBI Official Synonym Full Names: mediator complex subunit 13
• NCBI Official Symbol: MED13
• NCBI Official Synonym Symbols: ARC250; THRAP1; DRIP250; HSPC221; TRAP240
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 13; thyroid hormone receptor associated protein 1; thyroid hormone receptor-associated protein 1; activator-recruited cofactor 250 kDa component; thyroid hormone receptor-associated protein, 240 kDa subu
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 13
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED13
• UniProt Synonym Gene Names: ARC250; KIAA0593; THRAP1; TRAP240; ARC250; Trap240; DRIP250
• UniProt Entry Name: MED13_HUMAN

NCBI Description

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008]

Uniprot Description

Trap240: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 13 family.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 17q22-q23

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function: ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; transcription cofactor activity; receptor activity; thyroid hormone receptor binding

Biological Process: steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter