Western Blot (WB)
(Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Mouse Med12 Polyclonal Antibody | anti-Med12 antibody
Mouse Med12 Antibody (C-term)
Western Blot (WB)
(Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Med12 Antibody (C-term)at 1:1000 dilution + mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Med12 Antibody (C-term)at 1:1000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-Med12 Antibody (C-term)at 1:1000 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Med12 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: C2C12 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Med12 Antibody (C-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: C2C12 whole cell lysatesLane 3: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 245 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Philibert R.A.,et al.Mol. Psychiatry 3:303-309(1998).
Okazaki N.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Park J.,et al.Mol. Cell 50:919-930(2013).
NCBI and Uniprot Product Information
Uniprot Description
MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator
Cellular Component: nucleoplasm; centrosome; membrane; nucleolus; Srb-mediator complex; nucleus; ubiquitin ligase complex
Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; transcription coactivator activity; transcription cofactor activity; beta-catenin binding; receptor activity; chromatin binding; thyroid hormone receptor binding; transcription factor binding
Biological Process: steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; Schwann cell development; negative regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; heart development; stem cell maintenance; protein ubiquitination; Wnt receptor signaling pathway through beta-catenin; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; neural tube closure; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; oligodendrocyte development
Research Articles on Med12
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Product Notes
The Med12 med12 (Catalog #AAA9215088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Med12 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Med12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:2000. Researchers should empirically determine the suitability of the Med12 med12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Med12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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