Western Blot (WB)
(MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).)
Rabbit MeCP2 Polyclonal Antibody | anti-MECP2 antibody
MeCP2 Antibody (N-term S80)
Western Blot (WB)
(MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).)
Western Blot (WB)
(MeCP2 Antibody (pS80) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).)
Immunohistochemistry (IHC)
(MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MeCP2 Antibody (N-term S80) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MeCP2 Antibody (N-term S80) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MeCP2 Antibody (N-term S80) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. In contrast to other MBD family members, MECP2 is
X-linked and subject to X inactivation. MECP2 is dispensible in
stem cells, but is essential for embryonic development. MECP2 gene
mutations are the cause of most cases of Rett syndrome, a
progressive neurologic developmental disorder and one of the most
common causes of mental retardation in females. [provided by
RefSeq].
Pintaudi, M., et al. Epilepsy Behav (2010) In press :
Jain, D., et al. Pediatr. Neurol. 43(1):35-40(2010)
Harvey, C.G., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (3), 355-360 (2007) :
Francke, U. Nat Clin Pract Neurol 2(4):212-221(2006)
NCBI and Uniprot Product Information
NCBI Description
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]
Uniprot Description
MECP2: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Interacts with FNBP3. Interacts with CDKL5. Present in all adult somatic tissues tested. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: nucleoplasm; heterochromatin; extracellular space; cytosol; nucleus
Molecular Function: mRNA binding; protein domain specific binding; protein binding; siRNA binding; DNA binding; methyl-CpG binding; double-stranded methylated DNA binding; protein N-terminus binding; chromatin binding; transcription factor activity; transcription factor binding; transcription corepressor activity
Biological Process: catecholamine secretion; genetic imprinting; behavioral fear response; startle response; adult locomotory behavior; positive regulation of transcription, DNA-dependent; chromatin silencing; pathogenesis; neurological control of breathing; negative regulation of transcription from RNA polymerase II promoter; proprioception; sensory perception of pain; negative regulation of histone methylation; neuron maturation; negative regulation of histone acetylation; phosphatidylcholine metabolic process; post-embryonic development; protein localization; synaptogenesis; positive regulation of cell proliferation; dendrite development; cerebellum development; visual learning; negative regulation of neuron apoptosis; histone acetylation; mitochondrial electron transport, ubiquinol to cytochrome c; transcription, DNA-dependent; ventricular system development; glutamine metabolic process; social behavior; respiratory gaseous exchange; cardiolipin metabolic process; histone methylation; glucocorticoid metabolic process; positive regulation of synaptogenesis; regulation of systemic arterial blood pressure by neurological process; long-term memory; inositol metabolic process; response to hypoxia; regulation of excitatory postsynaptic membrane potential; negative regulation of transcription, DNA-dependent
Disease: Rett Syndrome; Mental Retardation, X-linked, Syndromic 13; Autism, Susceptibility To, X-linked 3; Lubs X-linked Mental Retardation Syndrome; Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations; Angelman Syndrome
Research Articles on MECP2
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Product Notes
The MECP2 mecp2 (Catalog #AAA9205285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 58-87. The MeCP2 Antibody (N-term S80) reacts with Human (Predicted Reactivity: Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MeCP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the MECP2 mecp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MeCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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