Western Blot (WB)
(Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (E11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)
Rabbit anti-Human MeCP2 Polyclonal Antibody | anti-MeCP2 antibody
MeCP2, NT (MECP2, Methyl-CpG-binding protein 2) (FITC)
Gene Names
MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MeCP2; Polyclonal Antibody; NT (MECP2; Methyl-CpG-binding protein 2) (FITC); MECP2; Methyl-CpG-binding protein 2; anti-MeCP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-MeCP2 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3~33 amino acids from the N-terminal region of human MeCP2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (E11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)
Western Blot (WB)
(Western blot analysis of MeCP2 (arrow) using rabbit polyclonal MeCP2 Antibody (E11). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MeCP2 gene.)
Flow Cytometry (FC/FACS)
(MeCP2 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MeCP2 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MeCP2 antibody
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Product Categories/Family for anti-MeCP2 antibody
References
dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005). Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005). Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004). Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004). Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
486
NCBI Official Full Name
methyl-CpG-binding protein 2 isoform 2
NCBI Official Synonym Full Names
methyl CpG binding protein 2 (Rett syndrome)
NCBI Official Symbol
MECP2
NCBI Official Synonym Symbols
RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
NCBI Protein Information
methyl-CpG-binding protein 2; meCp-2 protein
UniProt Protein Name
Methyl-CpG-binding protein 2
Protein Family
UniProt Gene Name
MECP2
UniProt Synonym Gene Names
MeCp-2 protein; MeCp2
UniProt Entry Name
MECP2_HUMAN
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Product Notes
The MeCP2 mecp2 (Catalog #AAA6319905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MeCP2, NT (MECP2, Methyl-CpG-binding protein 2) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MeCP2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MeCP2 mecp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MeCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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