Rabbit MDM2 Polyclonal Antibody | anti-MDM2 antibody

MDM2 (F414) Polyclonal Antibody

Cat. Num. AAA3000688

• Gene Names: MDM2; HDMX; LSKB; hdm2; ACTFS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MDM2; Polyclonal Antibody; MDM2 (F414) Polyclonal Antibody; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; anti-MDM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MDM2 (F414) polyclonal antibody detects endogenous levels of MDM2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 497

• Applicable Applications for anti-MDM2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MDM2 (F414) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MDM2 (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of MDM2 (F414) pAb at 1:500 dilutionLane1:HCC827 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug))

Related Product Information for anti-MDM2 antibody

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 89993689
• NCBI GeneID: 4193
• NCBI Accession #: NP_002383.2
• NCBI GenBank Nucleotide #: NP_002383.2
• UniProt Accession #: Q00987
• Molecular Weight: ~ 55, 90kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2 isoform a
• NCBI Official Synonym Full Names: MDM2 proto-oncogene
• NCBI Official Symbol: MDM2
• NCBI Official Synonym Symbols: HDMX; LSKB; hdm2; ACTFS
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MDM2
• UniProt Synonym Gene Names: Hdm2
• UniProt Entry Name: MDM2_HUMAN

NCBI Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Oncoprotein; Inhibitor; Ubiquitin conjugating system; Nucleolus

Chromosomal Location of Human Ortholog: 12q14.3-q15

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; nucleolus; plasma membrane; synapse; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; response to morphine; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to carbohydrate stimulus; response to antibiotic; positive regulation of protein export from nucleus; synaptic transmission; negative regulation of DNA damage response, signal transduction by p53 class mediator; response to magnesium ion; establishment of protein localization; peptidyl-lysine modification; positive regulation of cell proliferation; protein complex assembly; response to iron ion; epidermal growth factor receptor signaling pathway; response to drug; traversing start control point of mitotic cell cycle; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein destabilization; response to ether; response to cocaine; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; innate immune response; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Accelerated Tumor Formation, Susceptibility To