Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS7601306 (MDH1 Antibody) at dilution of 1:100)
Rabbit MDH1 Polyclonal Antibody | anti-MDH1 antibody
MDH1 Rabbit Polyclonal
Immunogen Affinity Purified
Immunogen Affinity Purified
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS7601306 (MDH1 Antibody) at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using MBS7601306 (MDH1 Antibody) at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using MDH1 antibody MBS7601306 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells, using MDH1 antibody MBS7601306 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red) .)
Immunoprecipitation (IP)
(IP Result of anti-MDH1 (IP: MBS7601306, 4ug; Detection: MBS7601306 1:800) with mouse liver tissue lysate 6400ug.)
Immunoprecipitation (IP)
(IP Result of anti-MDH1 (IP: MBS7601306, 4ug; Detection: MBS7601306 1:800) with mouse liver tissue lysate 6400ug.)
Western Blot (WB)
(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7601306 (MDH1 antibody) at dilution of 1:500)
Western Blot (WB)
(HepG2 cells were subjected to SDS PAGE followed by western blot with MBS7601306 (MDH1 antibody) at dilution of 1:500)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]
Uniprot Description
MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]
Protein type: EC 1.1.1.96; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Oxidoreductase
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: centrosome; cytoplasm; cytosol; extracellular space; mitochondrion; myelin sheath
Molecular Function: diiodophenylpyruvate reductase activity; L-malate dehydrogenase activity; malic enzyme activity; NAD binding; protein binding
Biological Process: gluconeogenesis; malate metabolic process; NADH metabolic process; oxaloacetate metabolic process; positive regulation of defense response to virus by host; tricarboxylic acid cycle
Research Articles on MDH1
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Product Notes
The MDH1 mdh1 (Catalog #AAA7601306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MDH1 Rabbit Polyclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MDH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the MDH1 mdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MDH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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