CD86 active protein

CD86 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123335

• Gene Names: Cd86; B7-2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD86; CD86 Protein; Rat; Recombinant (His Tag); Rat CD86/B7-2 Protein (His Tag); CD86 antigen; CD86 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile250

• Species: Rat
• Activity: Measured by its binding ability in a functional ELISA. Immobilized rat CD86 at 2ug/ml (100uL/well) can bind mouse CD28 with a linear range of 6.4-160ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Pro 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD86 active protein

Background: CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Product Categories/Family for CD86 active protein

Cancer Drug Targets

References

Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.

NCBI and Uniprot Product Information

• NCBI GI #: 11464989
• NCBI GeneID: 56822
• NCBI Accession #: NP_064466.1
• NCBI GenBank Nucleotide #: NM_020081.1
• UniProt Accession #: O35531; F1LNG0
• Molecular Weight: 35,574 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD86
• NCBI Official Synonym Full Names: CD86 molecule
• NCBI Official Symbol: Cd86
• NCBI Official Synonym Symbols: B7-2
• NCBI Protein Information: T-lymphocyte activation antigen CD86
• UniProt Protein Name: CD86 molecule
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd86

NCBI Description

plays a role in T-cell activation and proliferation [RGD, Feb 2006]