Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat MDG1/DNAJB9 Polyclonal Antibody | anti-DNAJB9 antibody
Goat anti-MDG1/DNAJB9 Antibody
Gene Names
DNAJB9; MDG1; ERdj4; MDG-1; MST049; MSTP049
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MDG1/DNAJB9; Polyclonal Antibody; Goat anti-MDG1/DNAJB9 Antibody; Goat Anti-DNAJB9 (aa61-75) Antibody; DNAJB9; MDG1; ERdj4; DKFZP564F1862; DnaJ (Hsp40) homolog; subfamily B; member 9; endoplasmic reticulum DnaJ homolog 4; microvascular endothelial differentiation gene 1; MST049; MSTP049; DNAJB9 antibody; MDG1 antibody; ERdj4 antibody; DKFZP564F1862 antibody; member 9 antibody; endoplasmic reticulum DnaJ homolog 4 antibody; microvascular endothelial differentiation gene 1 antibody; MST049 antibody; MSTP049 antibody; MDG1 / DNAJB9; anti-DNAJB9 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RGNMVTTYTDCSGQ
Sequence Length
223
Applicable Applications for anti-DNAJB9 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: In paraffin embedded Human Kidney shows ER staining in PCT. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows ER staining in PCT. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1ug/ml.
Immunogen
Peptide with sequence RGNMVTTYTDCSGQ, from the C Terminus of the protein sequence according to NP_036460.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,518 Da
NCBI Official Full Name
dnaJ homolog subfamily B member 9
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member B9
NCBI Official Symbol
DNAJB9
NCBI Official Synonym Symbols
MDG1; ERdj4; MDG-1; MST049; MSTP049
NCBI Protein Information
dnaJ homolog subfamily B member 9
UniProt Protein Name
DnaJ homolog subfamily B member 9
Protein Family
UniProt Gene Name
DNAJB9
UniProt Synonym Gene Names
MDG1; ER-resident protein ERdj4; ERdj4; Mdg-1
UniProt Entry Name
DNJB9_HUMAN
NCBI Description
This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. [provided by RefSeq, Dec 2010]
Uniprot Description
DNAJB9: Acts as a co-chaperone with an Hsp70 protein.
Protein type: Endoplasmic reticulum; Chaperone
Chromosomal Location of Human Ortholog: 7q31|14q24.2-q24.3
Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum membrane; nucleolus
Molecular Function: misfolded protein binding; protein binding
Biological Process: ER-associated protein catabolic process
Research Articles on DNAJB9
Similar Products
Product Notes
The DNAJB9 dnajb9 (Catalog #AAA420281) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MDG1/DNAJB9 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MDG1/DNAJB9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Kidney shows ER staining in PCT. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1ug/ml. Researchers should empirically determine the suitability of the DNAJB9 dnajb9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RGNMVTTYTD CSGQ. It is sometimes possible for the material contained within the vial of "MDG1/DNAJB9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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