Rabbit anti-Human MCRS1 Polyclonal Antibody | anti-MCRS1 antibody

MCRS1, NT (MCRS1, INO80Q, MSP58, Microspherule protein 1, 58kD microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS2) (FITC)

Cat. Num. AAA6319774

• Gene Names: MCRS1; P78; MCRS2; MSP58; INO80Q; ICP22BP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCRS1; Polyclonal Antibody; NT (MCRS1; INO80Q; MSP58; Microspherule protein 1; 58kD microspherule protein; Cell cycle-regulated factor p78; INO80 complex subunit J; MCRS2) (FITC); MCRS2; anti-MCRS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 462

• Applicable Applications for anti-MCRS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCRS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human MCRS1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCRS1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MCRS1 antibody detected the MCRS1 protein (arrow).)

Related Product Information for anti-MCRS1 antibody

MCRS1 modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity.

Product Categories/Family for anti-MCRS1 antibody

Antibodies; Transcription Factors

References

Lin, W., et al. J. Cell. Mol. Med. 13 (11-12), 4608-4622 (2009): Shi, H., et al. Cancer Sci. 100(9):1585-1590(2009). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Wu, J.L., et al. BMC Cell Biol. 10, 9 (2009): Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 29893564
• NCBI GeneID: 10445
• NCBI Accession #: NP_006328.2; NP_001012300.1
• NCBI GenBank Nucleotide #: NP_006328.2
• UniProt Accession #: Q96EZ8
• NCBI Official Full Name: microspherule protein 1 isoform 1
• NCBI Official Synonym Full Names: microspherule protein 1
• NCBI Official Symbol: MCRS1
• NCBI Official Synonym Symbols: P78; MCRS2; MSP58; INO80Q; ICP22BP
• NCBI Protein Information: microspherule protein 1
• UniProt Protein Name: Microspherule protein 1
• Protein Family: Microspherule protein
• UniProt Gene Name: MCRS1
• UniProt Synonym Gene Names: INO80Q; MSP58
• UniProt Entry Name: MCRS1_HUMAN

Uniprot Description

Function: Modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. May also be an inhibitor of TERT telomerase activity. Ref.3 Ref.5 Ref.15

Subunit structure: Binds to NOP2, DAXX, PINX1, TERT and Herpes simplex virus ICP22. Interacts with CCDC85B. Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the N-terminus of INO80. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Ref.1 Ref.2 Ref.3 Ref.6 Ref.7 Ref.8 Ref.10 Ref.15 Ref.17

Subcellular location: Nucleus. Nucleus › nucleolus. Note: In microspherules in the nucleolus. Ref.1 Ref.3 Ref.10 Ref.15

Tissue specificity: Detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes.

Developmental stage: Cell-cycle regulated: levels are highest early in S phase; not detectable in G2.

Sequence similarities: Contains 1 FHA domain.

Sequence caution: The sequence AAC68599.1 differs from that shown. Reason: Frameshift at several positions.