Rabbit anti-Human MCOLN3 Polyclonal Antibody | anti-MCOLN3 antibody

MCOLN3, CT (MCOLN3, Mucolipin-3) (Biotin)

Cat. Num. AAA6319751

• Gene Names: MCOLN3; TRPML3; TRP-ML3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCOLN3; Polyclonal Antibody; CT (MCOLN3; Mucolipin-3) (Biotin); Mucolipin-3; anti-MCOLN3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 553

• Applicable Applications for anti-MCOLN3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCOLN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-546 amino acids from the C-terminal region of human MCOLN3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCOLN3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MCOLN3 antibody detected the MCOLN3 protein (arrow).)

Related Product Information for anti-MCOLN3 antibody

Mucolipins constitute a family of cation channel proteins with homologs in mouse, Drosophila, and C. elegans. Mutations in the human MCOLN1 gene (MIM 605248) cause mucolipodosis IV (MIM 262650).

Product Categories/Family for anti-MCOLN3 antibody

Antibodies; Proteins

References

Kim, H.J., et al. J. Biol. Chem. 285(22):16513-16520(2010). Curcio-Morelli, C., et al. J. Cell. Physiol. 222(2):328-335(2010). Kim, H.J., et al. Traffic 10(8):1157-1167(2009). Martina, J.A., et al. Traffic 10(8):1143-1156(2009). Grimm, C., et al. J. Biol. Chem. 284(20):13823-13831(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 24496763
• NCBI GeneID: 55283
• NCBI Accession #: NP_060768.8
• NCBI GenBank Nucleotide #: NP_060768.8
• UniProt Accession #: Q8TDD5
• NCBI Official Full Name: mucolipin-3 isoform 1
• NCBI Official Synonym Full Names: mucolipin 3
• NCBI Official Symbol: MCOLN3
• NCBI Official Synonym Symbols: TRPML3; TRP-ML3
• NCBI Protein Information: mucolipin-3
• UniProt Protein Name: Mucolipin-3
• Protein Family: Mucolipin
• UniProt Gene Name: MCOLN3
• UniProt Synonym Gene Names: TRPML31 PublicationManual assertion based on opinion iniRef.4

NCBI Description

This gene encodes one of members of the mucolipin cation channel proteins. Mutation studies of the highly similar protein in mice have shown that the protein is found in cochlea hair cells, and mutant mice show early-onset hearing loss and balance problems. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Nonselective ligand-gated cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca2+-permeable cation channel with inwardly rectifying activity (PubMed:18369318, PubMed:19497048, PubMed:19522758, PubMed:19885840, PubMed:29106414). Mediates release of Ca2+ from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway (PubMed:21245134). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth (). Involved in the regulation of autophagy (PubMed:19522758). Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca2+ for the fusion process (). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events (PubMed:19885840). Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3 (PubMed:23469151).