Rabbit anti-Human MCM7 Polyclonal Antibody | anti-MCM7 antibody

MCM7, NT (MCM7, CDC47, MCM2, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3) (Azide free) (HRP)

Cat. Num. AAA6319720

• Gene Names: MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCM7; Polyclonal Antibody; NT (MCM7; CDC47; MCM2; DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3) (Azide free) (HRP); P1.1-MCM3; anti-MCM7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 543

• Applicable Applications for anti-MCM7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCM7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-142 amino acids from the N-terminal region of human MCM7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCM7 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MCM7 antibody detected the MCM7 protein (arrow).)

Related Product Information for anti-MCM7 antibody

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Categories/Family for anti-MCM7 antibody

Antibodies; Cell Division Cycle (CDC)

References

Lau, K.M., et al. Oncogene 29(40):5475-5489(2010). Kim, D.W., et al. Mol. Biochem. Parasitol. 173(1):10-16(2010). Olson, J.E., et al. Breast Cancer Res. Treat. (2010). In press: Rojiani, M.V., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):278-282(2010). Poliseno, L., et al. Sci Signal 3 (117), RA29 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 33469922
• NCBI GeneID: 4176
• NCBI Accession #: NP_877577.1; NP_005907.3
• NCBI GenBank Nucleotide #: NP_877577.1
• UniProt Accession #: P33993
• NCBI Official Full Name: DNA replication licensing factor MCM7 isoform 2
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 7
• NCBI Official Symbol: MCM7
• NCBI Official Synonym Symbols: MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3
• NCBI Protein Information: DNA replication licensing factor MCM7
• UniProt Protein Name: DNA replication licensing factor MCM7
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM7
• UniProt Synonym Gene Names: CDC47; MCM2
• UniProt Entry Name: MCM7_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]