Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCM4 antibody. (1: NIH/3T3 cell lysate 2: Hela cell lysate 3: K562 cell lysate))
Rabbit MCM4 Polyclonal Antibody | anti-MCM4 antibody
MCM4 (Mini Chromosome Maintenance Protein 4, CDC21, CDC54, hCdc21, P1-CDC21) (AP)
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCM4 antibody. (1: NIH/3T3 cell lysate 2: Hela cell lysate 3: K562 cell lysate))
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MCM4 antibody. (1: NIH/3T3 cell lysate 2: Hela cell lysate 3: K562 cell lysate))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human brain tissue using MCM4 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human brain tissue using MCM4 antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
MCM4: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex.
Protein type: EC 3.6.4.12; DNA replication
Chromosomal Location of Human Ortholog: 8q11.2
Cellular Component: nucleoplasm; membrane; MCM complex; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding; single-stranded DNA binding; ATP binding
Biological Process: DNA unwinding during replication; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
Disease: Natural Killer Cell And Glucocorticoid Deficiency With Dna Repair Defect
Research Articles on MCM4
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Product Notes
The MCM4 mcm4 (Catalog #AAA6426287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM4 (Mini Chromosome Maintenance Protein 4, CDC21, CDC54, hCdc21, P1-CDC21) (AP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MCM4 mcm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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