Western Blot (WB)
(Western Blot analysis of K562, Jurkat and hela cell using MCM3 Polyclonal Antibody at dilution of 1:550)
Rabbit MCM3 Polyclonal Antibody | anti-MCM3 antibody
MCM3 Polyclonal Antibody
Gene Names
mcm3; xmcm3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
MCM3; Polyclonal Antibody; MCM3 Polyclonal Antibody; HCC5; P1.h; RLFB; P1-MCM3; anti-MCM3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
0.8mg/mL (varies by lot)
Sequence Length
807
Applicable Applications for anti-MCM3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human MCM3
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of K562, Jurkat and hela cell using MCM3 Polyclonal Antibody at dilution of 1:550)
Western Blot (WB)
(Western Blot analysis of K562, Jurkat and hela cell using MCM3 Polyclonal Antibody at dilution of 1:550)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human liver cancer using MCM3 Polyclonal Antibody at dilution of 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human liver cancer using MCM3 Polyclonal Antibody at dilution of 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human ovarian cancer using MCM3 Polyclonal Antibody at dilution of 1:30)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human ovarian cancer using MCM3 Polyclonal Antibody at dilution of 1:30)
Related Product Information for anti-MCM3 antibody
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
90,405 Da
NCBI Official Full Name
maternal DNA replication licensing factor mcm3
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3<
NCBI Official Symbol
mcm3
NCBI Official Synonym Symbols
xmcm3
NCBI Protein Information
maternal DNA replication licensing factor mcm3; P1 homolog; XRLF subunit beta; mMCM3; maternal Mcm3 protein; maternal minichromosome maintenance protein 3; p100
UniProt Protein Name
Maternal DNA replication licensing factor mcm3
Protein Family
UniProt Gene Name
mmcm3
UniProt Synonym Gene Names
mMCM3; xMCM3
UniProt Entry Name
MCM3M_XENLA
Uniprot Description
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. The existence of maternal and zygotic forms of mcm3 and mcm6 suggests that specific forms of mcm2-7 complexes may be used during different stages of development.
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Product Notes
The MCM3 mmcm3 (Catalog #AAA2519937) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCM3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MCM3 mmcm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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