Rabbit anti-Human, Mouse MBTPS2 Polyclonal Antibody | anti-MBTPS2 antibody

MBTPS2 Polyclonal Antibody

Cat. Num. AAA3007266

• Gene Names: MBTPS2; S2P; IFAP; KFSD; OI19; KFSDX; OLMSX; BRESEK
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MBTPS2; Polyclonal Antibody; MBTPS2 Polyclonal Antibody; Membrane-bound transcription factor site-2 protease (EC: 3.4.24.85); Endopeptidase S2P; Sterol regulatory element-binding proteins intramembrane protease; SREBPs intramembrane protease; S2P; anti-MBTPS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MBTPS2 polyclonal antibody detects endogenous levels of MBTPS2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 519

• Applicable Applications for anti-MBTPS2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MBTPS2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MBTPS2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysateLane2:RAW264. 7 whole cell lysate)

Related Product Information for anti-MBTPS2 antibody

The transcription factors SREBPs (sterol regulatory element binding proteins) span the ER membrane and, in response to sterol depletion, the N-terminal domain of SREBPs are proteolytically activated, released from the membrane and then translocate to the nucleus where they induce the expression of genes regulating cholesterol metabolism. This proteolytic activation requires the sequential cleavage of SREBPs at Site-1, within the lumen of the ER, followed by cleavage at Site-2, within the first transmembrane domain. The cleavage at Site-1 separates the N-terminal and C-terminal domains of the protein and it requires the serine protease, S1P (Site-1 protease). Site-2 is subsequently processed by a putative zinc metalloprotease S2P, which releases the activated N-terminal domain for nuclear translocation. This proteolytic pathway is tightly regulated by sterol levels and is under the control of SCAP (SREBP cleavage-activating protein). SCAP, a sterol sensor, is latently bound to the C-terminal regulatory domains of the SREBPs, and it regulates cleavage of SREBPs at Site-1. Sterol levels influence the activity of SCAP, as SCAP is activated only in sterol-depleted cells and it inhibited by sterol accumulation.

NCBI and Uniprot Product Information

• NCBI GI #: 7706693
• NCBI GeneID: 51360
• NCBI Accession #: NP_056968.1
• NCBI GenBank Nucleotide #: NP_056968.1
• UniProt Accession #: O43462
• Molecular Weight: ~ 57kDa
• NCBI Official Full Name: membrane-bound transcription factor site-2 protease
• NCBI Official Synonym Full Names: membrane bound transcription factor peptidase, site 2
• NCBI Official Symbol: MBTPS2
• NCBI Official Synonym Symbols: S2P; IFAP; KFSD; OI19; KFSDX; OLMSX; BRESEK
• NCBI Protein Information: membrane-bound transcription factor site-2 protease
• UniProt Protein Name: Membrane-bound transcription factor site-2 protease
• Protein Family: Membrane-bound transcription factor site-2 protease
• UniProt Gene Name: MBTPS2
• UniProt Synonym Gene Names: S2P; SREBPs intramembrane protease
• UniProt Entry Name: MBTP2_HUMAN

NCBI Description

This gene encodes a intramembrane zinc metalloprotease, which is essential in development. This protease functions in the signal protein activation involved in sterol control of transcription and the ER stress response. Mutations in this gene have been associated with ichthyosis follicularis with atrichia and photophobia (IFAP syndrome); IFAP syndrome has been quantitatively linked to a reduction in cholesterol homeostasis and ER stress response.[provided by RefSeq, Aug 2009]

Uniprot Description

MBTPS2: Intramembrane proteolysis of sterol-regulatory element- binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop. Expressed in heart, brain, placenta, lung, liver, muscle, kidney and pancreas. Belongs to the peptidase M50A family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Protease; EC 3.4.24.85

Chromosomal Location of Human Ortholog: Xp22.12-p22.11

Cellular Component: Golgi membrane; cytoplasm; integral to membrane

Molecular Function: metalloendopeptidase activity; metal ion binding

Biological Process: cholesterol metabolic process; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; membrane protein intracellular domain proteolysis; unfolded protein response; positive regulation of transcription factor activity

Disease: Ifap Syndrome With Or Without Bresheck Syndrome; Palmoplantar Keratoderma, Mutilating, With Periorificial Keratotic Plaques, X-linked; Keratosis Follicularis Spinulosa Decalvans, X-linked