Western Blot (WB)
(MBD6 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MBD6 antibody detected the MBD6 protein (arrow).)
Rabbit anti-Mouse, Human MBD6 Polyclonal Antibody | anti-MBD6 antibody
MBD6 Antibody (C-term)
Reactivity
Mouse, Human
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MBD6; Polyclonal Antibody; MBD6 Antibody (C-term); Methyl-CpG-binding domain protein 6; Methyl-CpG-binding protein MBD6; KIAA1887; anti-MBD6 antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MBD6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 977-1003 amino acids from the C-terminal region of human MBD6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
977-1003
Sequence Length
1003
Applicable Applications for anti-MBD6 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Cellular Location
Nucleus. Chromosome. Note = Associated with pericentric heterochromatin in about 25% ofthe cells
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(MBD6 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MBD6 antibody detected the MBD6 protein (arrow).)
Western Blot (WB)
(MBD6 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MBD6 antibody detected the MBD6 protein (arrow).)
Western Blot (WB)
(All lanes : Anti-MBD6 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-MBD6 Antibody (C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: U-251 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 101 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MBD6 antibody
Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro).
References
Laget, S., et al. PLoS ONE 5 (8), E11982 (2010) :
Roloff, T.C., et al. BMC Genomics 4 (1), 1 (2003) :
Roloff, T.C., et al. BMC Genomics 4 (1), 1 (2003) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101201
NCBI Official Full Name
methyl-CpG-binding domain protein 6
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 6
NCBI Official Symbol
MBD6
NCBI Protein Information
methyl-CpG-binding domain protein 6
UniProt Protein Name
Methyl-CpG-binding domain protein 6
Protein Family
UniProt Gene Name
MBD6
UniProt Synonym Gene Names
KIAA1887
UniProt Entry Name
MBD6_HUMAN
Uniprot Description
MBD6: Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro).
Chromosomal Location of Human Ortholog: -
Cellular Component: nucleoplasm; nucleolus; nucleus; chromocenter
Molecular Function: DNA binding; chromatin binding
Research Articles on MBD6
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Product Notes
The MBD6 mbd6 (Catalog #AAA9215021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 977-1003. The MBD6 Antibody (C-term) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's MBD6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the MBD6 mbd6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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