Rabbit anti-Human MBD4 Polyclonal Antibody | anti-MBD4 antibody

Anti-MBD4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100658

• Gene Names: MBD4; MED1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MBD4; Polyclonal Antibody; Anti-MBD4 Antibody; Rabbit Polyclonal; MBD4 Antibody; Rabbit PAb; Antigen Affinity Purified; methyl-CpG binding domain 4 DNA glycosylase; Anti-MED1 Antibody; anti-MBD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MBD4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MBD4 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.1-0.5uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human MBD4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human MBD4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(MBD4 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MBD4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-MBD4 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 66 kDaObserved band size: 53 kDa)

Western Blot (WB)

(Anti-MBD4 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: K562 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:66 kDaObserved band size:53 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MBD4 antibody

MBD4 is the only methyl-CpG binding protein that possesses a C-terminal glycosylase domain. MBD4 contains a methyl-CpG-binding domain which provides the localization of the enzyme at the CpG sites, and a DNA glycosylase domain that is responsible for the catalytic activity. Methyl-CpG binding domain protein 4 (MBD4) is a DNA glycosylase that can remove 5-fluorodeoxyuracil from DNA as well as repair T:G or U:G mismatches. MBD4 is a target for frameshift mutation with DNA mismatch repair (MMR) deficiency, creating a truncated MBD4 protein (TruMBD4) that lacks its glycosylase domain. MBD4 (methyl-CpG binding domain protein 4) is an important G: T glycosylase that can identify T-G mismatches. It plays a role in active demethylation through base excision repair. Overexpression of MBD4 gene can cause the demethylation of numerous genes, and the remethylation of MBD4-associated genes can occur when the MBD4 gene is knocked out.

NCBI and Uniprot Product Information

• NCBI GI #: 50400819
• NCBI GeneID: 8930
• UniProt Accession #: O95243; Q2MD36; Q7Z4T3; Q96F09; B4DZN2; D3DNC3; D3DNC4; E9PEE4
• Molecular Weight: 580
• NCBI Official Full Name: Methyl-CpG-binding domain protein 4
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 4
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4; G/T mismatch glycosylase; G/U mismatch glycosylase; methyl-CpG-binding protein MBD4; 3,N(4)-ethenocytosine glycosylase; methyl-CpG-binding endonuclease 1; mismatch-specific DNA N-glycosylase; putative methyl-CpG bindin
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Apoptosis; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; chromatin

Molecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activity

Biological Process: response to radiation; base-excision repair, AP site formation; DNA damage response, signal transduction resulting in induction of apoptosis; depyrimidination; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic