Rabbit anti-Human MBD4 Polyclonal Antibody | anti-MBD4 antibody

MBD4 (Methyl-CpG-binding Domain 4) (PE)

Cat. Num. AAA6319319

• Gene Names: MBD4; MED1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MBD4; Polyclonal Antibody; MBD4 (Methyl-CpG-binding Domain 4) (PE); Methyl-CpG-binding Domain 4; anti-MBD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MBD4 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from a portion within aa 268~281 of human MBD4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

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Related Product Information for anti-MBD4 antibody

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and the MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.

Product Categories/Family for anti-MBD4 antibody

Antibodies; DNA Replication; Repair

References

Evertson, S., et al., Anticancer Res. 23(4):3569-3574 (2003). Yamada, T., et al., Cancer Lett. 181(1):115-120 (2002). Schlegel, J., et al., Oncol. Rep. 9(2):393-395 (2002). Petronzelli, F., et al., J. Biol. Chem. 275(42):32422-32429 (2000). Petronzelli, F., et al., J. Cell. Physiol. 185(3):473-480 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4505121
• NCBI GeneID: 8930
• NCBI Accession #: NP_003916
• NCBI GenBank Nucleotide #: NM_003925
• UniProt Accession #: O95243
• Molecular Weight: 66kDa
• NCBI Official Full Name: methyl-CpG-binding domain protein 4 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain 4, DNA glycosylase
• NCBI Official Symbol: MBD4
• NCBI Official Synonym Symbols: MED1
• NCBI Protein Information: methyl-CpG-binding domain protein 4
• UniProt Protein Name: Methyl-CpG-binding domain protein 4
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD4
• UniProt Synonym Gene Names: MED1
• UniProt Entry Name: MBD4_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MBD4: Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Apoptosis; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; chromatin

Molecular Function: protein binding; satellite DNA binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; endodeoxyribonuclease activity

Biological Process: response to radiation; base-excision repair, AP site formation; DNA damage response, signal transduction resulting in induction of apoptosis; depyrimidination; base-excision repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA repair; DNA catabolic process, endonucleolytic