Western Blot (WB)
(GCDFP-15 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GCDFP-15 antibody detected the GCDFP-15 protein (arrow).)
Rabbit anti-Human GCDFP-15 Polyclonal Antibody | anti-PIP antibody
GCDFP-15 Antibody (C-term)
Gene Names
PIP; GPIP4; GCDFP15; GCDFP-15
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GCDFP-15; Polyclonal Antibody; GCDFP-15 Antibody (C-term); Prolactin-inducible protein; Gross cystic disease fluid protein 15; Prolactin-induced protein; Secretory actin-binding protein; SABP; gp17; PIP; GCDFP15; GPIP4; anti-PIP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GCDFP-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the C-terminal region of human GCDFP-15.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.25 (varies by lot)
Sequence Positions
77-106
Sequence Length
146
Applicable Applications for anti-PIP antibody
Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(GCDFP-15 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GCDFP-15 antibody detected the GCDFP-15 protein (arrow).)
Western Blot (WB)
(GCDFP-15 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GCDFP-15 antibody detected the GCDFP-15 protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GCDFP-15 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of GCDFP-15 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(GCDFP-15 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCDFP-15 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(GCDFP-15 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCDFP-15 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-PIP antibody
Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
Product Categories/Family for anti-PIP antibody
References
Bryborn, M., et al. Allergy 65(2):220-228(2010)
Wang, L.J., et al. Appl. Immunohistochem. Mol. Morphol. 17(6):505-511(2009)
Yan, Z., et al. Diagn. Cytopathol. 37(7):475-478(2009)
Debily, M.A., et al. PLoS ONE 4 (3), E4696 (2009) :
Hassan, M.I., et al. J. Mol. Biol. 384(3):663-672(2008)
Wang, L.J., et al. Appl. Immunohistochem. Mol. Morphol. 17(6):505-511(2009)
Yan, Z., et al. Diagn. Cytopathol. 37(7):475-478(2009)
Debily, M.A., et al. PLoS ONE 4 (3), E4696 (2009) :
Hassan, M.I., et al. J. Mol. Biol. 384(3):663-672(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16572
NCBI Official Full Name
prolactin-inducible protein
NCBI Official Synonym Full Names
prolactin-induced protein
NCBI Official Symbol
PIP
NCBI Official Synonym Symbols
GPIP4; GCDFP15; GCDFP-15
NCBI Protein Information
prolactin-inducible protein
UniProt Protein Name
Prolactin-inducible protein
Protein Family
UniProt Gene Name
PIP
UniProt Synonym Gene Names
GCDFP15; GPIP4; GCDFP-15; SABP
UniProt Entry Name
PIP_HUMAN
Uniprot Description
PIP: Belongs to the PIP family.
Protein type: Secreted; Cytoskeletal; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q34
Cellular Component: extracellular space; apical plasma membrane; nucleus
Molecular Function: protein dimerization activity; protein binding; IgG binding; aspartic-type endopeptidase activity; actin binding; glycoprotein binding
Biological Process: retinal homeostasis; regulation of immune system process; detection of chemical stimulus involved in sensory perception of bitter taste; proteolysis
Research Articles on PIP
Similar Products
Product Notes
The PIP pip (Catalog #AAA9205244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 77-106. The GCDFP-15 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GCDFP-15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PIP pip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCDFP-15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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