Rabbit anti-Human MBD3L3 Polyclonal Antibody | anti-MBD3L3 antibody

MBD3L3 Antibody (C-term)

Cat. Num. AAA9211891

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MBD3L3; Polyclonal Antibody; MBD3L3 Antibody (C-term); Putative methyl-CpG-binding domain protein 3-like 3; MBD3-like protein 3; anti-MBD3L3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MBD3L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the C-terminal region of human MBD3L3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 156-184
• Sequence Length: 208

• Applicable Applications for anti-MBD3L3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(MBD3L3 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MBD3L3 antibody detected the MBD3L3 protein (arrow).)

Immunohistochemistry (IHC)

(MBD3L3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBD3L3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MBD3L3 Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(MBD3L3 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

References

Grimwood, J., et al. Nature 428(6982):529-535(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 256574745
• NCBI GeneID: 653657
• NCBI Accession #: NP_001157897.1
• NCBI GenBank Nucleotide #: NM_001164425.1
• UniProt Accession #: A6NE82
• Molecular Weight: 23095
• NCBI Official Full Name: putative methyl-CpG-binding domain protein 3-like 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3-like 3
• NCBI Official Symbol: MBD3L3
• NCBI Protein Information: putative methyl-CpG-binding domain protein 3-like 3
• UniProt Protein Name: Putative methyl-CpG-binding domain protein 3-like 3
• Protein Family: Putative methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3L3
• UniProt Synonym Gene Names: MBD3-like protein 3
• UniProt Entry Name: MB3L3_HUMAN

Uniprot Description

MBD3L3: Belongs to the MBD3L family

Chromosomal Location of Human Ortholog: 19p13.2

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent