Goat anti-Human MBD3 Polyclonal Antibody | anti-MBD3 antibody

MBD3 (Methyl CpG Binding Protein 3)

Cat. Num. AAA616915

• Gene Names: MBD3; MBD3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by human MBD-3 affinity chromatography.
• Synonyms: MBD3; Polyclonal Antibody; MBD3 (Methyl CpG Binding Protein 3); Anti -MBD3 (Methyl CpG Binding Protein 3); anti-MBD3 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Selected for its ability to recognize human MBD-3 in direct ELISAs and Western Blots.
• Purity/Purification: Affinity Purified; Purified by human MBD-3 affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

• Applicable Applications for anti-MBD3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 5 ng/lane under non-reducing and reducing conditions., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human MBD-3. The detection limit for rhMBD-3 is approximately 0.5 ng/well., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: E. coli-derived recombinant human MBD-3 isoform 2
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-MBD3 antibody

MBD-3 (methyl-CpG-binding domain protein # 3) is a 38-40kD member of the MBD family of transcriptional repressors. Through its MBD, and as part of a large complex of regulatory proteins, it binds methylated DNA, promoting gene silencing during S phase. Although it can homodimerize, it is only active when heterodimerized with MBD-2. Human MBD-3 is 291 amino acids (aa) in length, and contains an N-terminal methyl binding domain (aa's 1-72) and a C-terminal coiled-coil region (aa's 216-245) that may be involved in protein-protein interaction. Human MBD-3 shares 95% aa identity with mouse, rat, and canine MBD-3, with differences essentially limited to the C-terminal 25 amino acids.

Product Categories/Family for anti-MBD3 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505119
• NCBI GeneID: 53615
• NCBI Accession #: NP_003917.1
• NCBI GenBank Nucleotide #: NM_003926.5
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2; Q8WV36
• Molecular Weight: 32,844 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Official Synonym Symbols: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3; methyl-CpG-binding protein MBD3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3
• UniProt Entry Name: MBD3_HUMAN

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. [provided by RefSeq]

Uniprot Description

MBD3: methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

Protein type: Transcription, coactivator/corepressor; Gene silencing

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; heterochromatin; protein complex; cytoplasm; nuclear chromatin; NuRD complex

Molecular Function: protein binding; nucleosomal DNA binding; DNA binding; methyl-CpG binding

Biological Process: tissue development; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; methylation-dependent chromatin silencing; in utero embryonic development; ATP-dependent chromatin remodeling; negative regulation of transcription from RNA polymerase II promoter; histone acetylation