Rabbit MBD2 Polyclonal Antibody | anti-MBD2 antibody

Anti-MBD2 Antibody

Cat. Num. AAA8291740

• Gene Names: MBD2; DMTase; NY-CO-41
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by immunogen affinity chromatography
• Synonyms: MBD2; Polyclonal Antibody; Anti-MBD2 Antibody; Methyl-CpG-binding domain protein 2; Demethylase; DMTase; Methyl-CpG-binding protein MBD2; anti-MBD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of MBD2 protein.
• Purity/Purification: Purified by immunogen affinity chromatography
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 411

• Applicable Applications for anti-MBD2 antibody: Western Blot (WB)
• Application Notes: WB: 1/500 - 1/2000
• Immunogen: KLH-conjugated synthetic peptide of human MBD2
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MBD2 expression in Hela (A), mouse heart (B), rat brain (C) whole cell lysates.)

Related Product Information for anti-MBD2 antibody

Rabbit polyclonal antibody to MBD2

NCBI and Uniprot Product Information

• NCBI GI #: 4505117
• NCBI GeneID: 8932
• NCBI Accession #: NP_003918.1
• NCBI GenBank Nucleotide #: NM_003927.4
• UniProt Accession #: Q9UBB5; O95242; Q9UIS8
• Molecular Weight: 31,745 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 2
• NCBI Official Symbol: MBD2
• NCBI Official Synonym Symbols: DMTase; NY-CO-41
• NCBI Protein Information: methyl-CpG-binding domain protein 2
• UniProt Protein Name: Methyl-CpG-binding domain protein 2
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD2
• UniProt Synonym Gene Names: DMTase

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.